ArticleMolecular biotechnology2013
Increased understanding of the biochemistry and biosynthesis of MUC2 and other gel-forming mucins through the recombinant expression of their protein domains.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Dynamic glycan network engineering of native mucin enables reversible, self-healing, and adhesive hydrogel interfaces.Nanoscale advances · 2026Article
- Intestinal Mucin Glycosylation: Structural Regulation, Homeostasis Maintenance and Disease Association.Biomolecules · 2025Review
- Selective Biofilm Inhibition through Mucin-Inspired Engineering of Silk Glycopolymers.Journal of the American Chemical Society · 2024Article
- ThePolymers · 2024Review
- Recent Advancements in the Development of Nanocarriers for Mucosal Drug Delivery Systems to Control Oral Absorption.Pharmaceutics · 2023Review
- Bromelain and acetylcysteine (BromAcAmerican journal of cancer research · 2023Review
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- Development and Functional Properties of Intestinal Mucus Layer in Poultry.Frontiers in immunology · 2021Review
- A Microscopically Motivated Model for Particle Penetration into Swollen Biological Networks.Polymers · 2020Article
- Permeability of the small intestinal mucus for physiologically relevant studies: Impact of mucus location and ex vivo treatment.Scientific reports · 2019Article
- Engineering mucus to study and influence the microbiome.Nature reviews. Materials · 2019Article
- High level in vivo mucin-type glycosylation in Escherichia coli.Microbial cell factories · 2018Article
- Gut-microbiota-on-a-chip: an enabling field for physiological research.Microphysiological systems · 2018Article
- Dietary Nutrients, Proteomes, and Adhesion of Probiotic Lactobacilli to Mucin and Host Epithelial Cells.Microorganisms · 2018Review
- Role of mucins in lung homeostasis: regulated expression and biosynthesis in health and disease.Biochemical Society transactions · 2018Review
- Use of Recombinant Mucin Glycoprotein to Assess the Interaction of the Gastric Pathogen Helicobacter pylori with the Secreted Human Mucin MUC5AC.Bioengineering (Basel, Switzerland) · 2017Article
- Secondary Structure and Glycosylation of Mucus Glycoproteins by Raman Spectroscopies.Analytical chemistry · 2016Article
- Pathobiological implications of mucin glycans in cancer: Sweet poison and novel targets.Biochimica et biophysica acta · 2015Review
- Identification of PAM4 (clivatuzumab)-reactive epitope on MUC5AC: a promising biomarker and therapeutic target for pancreatic cancer.Oncotarget · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The gel-forming mucins are large and heavily O-glycosylated proteins which build up mucus gels. The recombinant production of full-length gel-forming mucins has not been possible to date. In order to study mucin biosynthesis and biochemistry, we and others have taken the alternative approach of constructing different recombinant proteins consisting of one or several domains of these large proteins and expressing them separately in different cell lines. Using this approach, we have determined that MUC2, the intestinal gel-forming mucin, dimerizes via its C-terminal cysteine-knot domain and also trimerizes via one of the N-terminal von Willebrand D domains. Both of these interactions are disulfide bond mediated. Via this assembly, a molecular network is built by which the mucus gel is formed. Here we discuss not only the functional understanding obtained from studies of the recombinant proteins, but also highlight the difficulties encountered when these proteins were produced recombinantly. We often found an accumulation of the proteins in the ER and consequently no secretion. This was especially apparent when the cysteine-rich domains of the N- and C-terminal parts of the mucins were expressed. Other proteins that we constructed were either not secreted or not expressed at all. Despite these problems, the knowledge of mucin biosynthesis and assembly has advanced considerably through the studies of these recombinant proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.