ArticleMarine biotechnology (New York, N.Y.)2013
Identification and characterization of an extracellular alkaline phosphatase in the marine diatom Phaeodactylum tricornutum.
Article in Marine biotechnology (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A plasma membrane Ca2+-dependent protein kinase PtCDPK2 promotes phosphorus starvation resilience in Phaeodactylum tricornutum.Journal of experimental botany · 2026Article
- Nitrogen status exerts dynamic control over phosphorus sensing and acquisition via PSR1 in colimited marine diatoms.Science advances · 2025Article
- Genetic engineering in diatoms: advances and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Validating a Promoter Library for Application in Plasmid-Based Diatom Genetic Engineering.ACS synthetic biology · 2023Article
- SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutum.Communications biology · 2021Article
- Biochemical and Molecular Aspects of Phosphorus Limitation in Diatoms and Their Relationship with Biomolecule Accumulation.Biology · 2021Review
- Mobilization and Cellular Distribution of Phosphate in the DiatomFrontiers in plant science · 2020Article
- Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation.Microbial cell factories · 2019Article
- Non-Conventional Metal Ion Cofactor Requirement of Dinoflagellate Alkaline Phosphatase and Translational Regulation by Phosphorus Limitation.Microorganisms · 2019Article
- Article
- The effects of phosphorus limitation on carbon metabolism in diatoms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Iron limitation of microbial phosphorus acquisition in the tropical North Atlantic.Nature communications · 2017Article
- Phosphorus Deficiency Inhibits Cell Division But Not Growth in the Dinoflagellate Amphidinium carterae.Frontiers in microbiology · 2016Article
- Rapidly diverging evolution of an atypical alkaline phosphatase (PhoA(aty)) in marine phytoplankton: insights from dinoflagellate alkaline phosphatases.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
10 authors.
Funding
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Abstract
In phosphorus-deficient conditions, Phaeodactylum tricornutum releases an alkaline phosphatase (PtAPase) to the medium that is readily detectable by activity staining. Nucleic acid and amino acid sequence of this alkaline phosphatase (APase) was identified by performing proteomic analysis and database searches. Sequence alignment suggests that PtAPase belongs to the PhoA family, and it possesses key residues at the Escherichia coli PhoA active site. Quantitative PCR results indicate that the induction of APase mRNA transcription is very sensitive to phosphorus availability and population growth. The molecular mass of native PtAPase (148 kDa) determined by gel filtration chromatography indicates that PtAPase, like most PhoA, is homodimeric. Zn and Mg ions are essential cofactors for most PhoA enzymes; however, PtAPase activity did not require Zn ions. In fact, 5 mM Zn²⁺, Mo²⁺, Co²⁺, Cd²⁺, or Cu²⁺ inhibited its enzymatic activity, whereas 5 mM Mn²⁺, Mg²⁺, or Ca²⁺ enhanced its enzymatic activity. The responses of PtAPase to divalent metal ions were different from those of most PhoAs, but were similar to the PhoA in a marine bacterium, Cobetia marina. Phylogenetic analysis shows that homologs of PhoA are also present in other diatom species, and that they clustered in a unique branch away from other PhoA members. PtAPase may represent a novel class of PhoA that helps diatoms to survive in the ocean. Quantification of the PtAPase mRNA may help monitor the physiological condition of diatoms in natural environments and artificial bioreactors.
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