ArticleLaboratory investigation; a journal of technical methods and pathology2013
Effect of the GPI anchor of human Thy-1 on antibody recognition and function.
Article in Laboratory investigation; a journal of technical methods and pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Soluble T-Cadherin: A Novel Biomarker for Fluid Resuscitation in Burn Patients-A Single-Center Retrospective Observational Study.Shock (Augusta, Ga.) · 2026Observational
- The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation.The Journal of biological chemistry · 2022Review
- A cell-based phenotypic library selection and screening approach for the de novo discovery of novel functional chimeric antigen receptors.Scientific reports · 2022Article
- Major Surface Antigens in ZoonoticPathogens (Basel, Switzerland) · 2022Review
- Identification and Clinical Associations of 3 Forms of Circulating T-cadherin in Human Serum.The Journal of clinical endocrinology and metabolism · 2021Observational
- Circulating tumor DNA as an emerging liquid biopsy biomarker for early diagnosis and therapeutic monitoring in hepatocellular carcinoma.International journal of biological sciences · 2020Review
- Thy-1 as an Integrator of Diverse Extracellular Signals.Frontiers in cell and developmental biology · 2019Review
- Redox signalling from NADPH oxidase targets metabolic enzymes and developmental proteins in Fusarium graminearum.Molecular plant pathology · 2019Article
- Thy-1, a Pathfinder Protein for the Post-genomic Era.Frontiers in cell and developmental biology · 2018Review
- Antigenic and immunogenic properties of chondrocytes. Implications for chondrocyte therapeutic transplantation and pathogenesis of inflammatory and degenerative joint diseases.Central-European journal of immunology · 2018Review
- Human embryonic stem cells extracellular vesicles and their effects on immortalized human retinal Müller cells.PloS one · 2018Article
- Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.Scientific reports · 2017Article
- Article
- Thy-1 interaction with Fas in lipid rafts regulates fibroblast apoptosis and lung injury resolution.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction.The Journal of cell biology · 2015Article
- Labeling Cell Surface GPIs and GPI-Anchored Proteins through Metabolic Engineering with Artificial Inositol Derivatives.Angewandte Chemie (International ed. in English) · 2015Article
- Thy1 (CD90) controls adipogenesis by regulating activity of the Src family kinase, Fyn.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Recent progress in synthetic and biological studies of GPI anchors and GPI-anchored proteins.Current opinion in chemical biology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Thymocyte differentiation antigen-1 (Thy-1) is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein expressed on numerous cell types, which regulates signals affecting cell adhesion, migration, differentiation, and survival. In addition, Thy-1 has been detected in the serum, cerebral spinal fluid, wound fluid from venous ulcers, synovial fluid from joints in rheumatoid arthritis, and, more recently, urine. We previously detected Thy-1 in the conditioned media of cytokine-stimulated lung fibroblasts, suggesting that Thy-1 shedding may be a response to cellular stress. Soluble and membrane-bound forms of Thy-1 from in vivo sources have been shown to be identical in size when deglycosylated, suggesting that soluble Thy-1 is separated from the diacyl glycerol portion of its GPI anchor by hydrolysis within the GPI moiety. For Thy-1- and other GPI-anchored proteins, delipidation induces a stable change in conformation that manifests itself in a change in antibody affinity for soluble forms. Using epitope-tagged recombinant soluble Thy-1, we report that widely available monoclonal antibodies to human Thy-1 are unable to detect soluble Thy-1 by immunoblotting. We re-evaluated the Thy-1 that we previously reported in the conditioned media of normal human lung fibroblasts and found it to be entirely insoluble. These findings suggest that most Thy-1 reported in body fluids retains its GPI anchor and may be associated with membrane fragments or vesicles. This phenomenon should be considered in the generation of antibodies and controls for Thy-1 bioassays. Furthermore, the changes in Thy-1 conformation with delipidation, beyond affecting antibody affinity, likely affect the ligand affinity and biological function of soluble vs released membrane-associated forms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.