ArticleDevelopmental biology2013
The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.
Article in Developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Critical role of Spatio-Temporally Regulated Maternal RNAs in Zebrafish Embryogenesis.bioRxiv : the preprint server for biology · 2024Article
- Functional annotation of a hugely expanded nanos repertoire in Lytechinus variegatus, the green sea urchin.Molecular reproduction and development · 2023Article
- Article
- Somatic cell conversion to a germ cell lineage: A violation or a revelation?Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.Developmental biology · 2021Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Molecular Cloning and Sexually Dimorphic Expression Analysis of nanos2 in the Sea Urchin, Mesocentrotus nudus.International journal of molecular sciences · 2019Article
- Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin.Methods in cell biology · 2019Review
- Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).Methods in cell biology · 2019Review
- A quiet space during rush hour: Quiescence in primordial germ cells.Stem cell research · 2017Article
- Transient translational quiescence in primordial germ cells.Development (Cambridge, England) · 2017Article
- Divergent Expression Patterns and Function Implications of Four nanos Genes in a Hermaphroditic Fish, Epinephelus coioides.International journal of molecular sciences · 2017Article
- Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.Developmental biology · 2016Article
- Germ Line Versus Soma in the Transition from Egg to Embryo.Current topics in developmental biology · 2015Review
- Expression profile of Nanos2 gene in dairy goat and its inhibitory effect on Stra8 during meiosis.Cell proliferation · 2014Article
- The biology of the germ line in echinoderms.Molecular reproduction and development · 2014Review
- Migration of sea urchin primordial germ cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Selective accumulation of germ-line associated gene products in early development of the sea star and distinct differences from germ-line development in the sea urchin.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Every which way--nanos gene regulation in echinoderms.Genesis (New York, N.Y. : 2000) · 2014Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus (Sp), and each nanos mRNA accumulates specifically in the small micromere (sMic) lineage. We found that a highly conserved element in the 3' UTR of nanos2 is sufficient for reporter expression selectively in the sMic lineage: microinjection into a Sp fertilized egg of an RNA that contains the GFP open reading frame followed by Sp nanos2 3'UTR leads to selective reporter enrichment in the small micromeres in blastulae. The same result was seen with nanos2 from the sea urchin Hemicentrotus pulcherrimus (Hp). In both species, the 5'UTR alone is not sufficient for the sMic localization but it always increased the sMic reporter enrichment when present with the 3'UTR. We defined an element conserved between Hp and Sp in the nanos2 3'UTR which is necessary and sufficient for protein enrichment in the sMic, and refer to it as GNARLE (Global Nanos Associated RNA Lability Element). We also found that the nanos2 3'UTR is essential for the selective RNA retention in the small micromeres; GNARLE is required but not sufficient for this process. These results show that a combination of selective RNA retention and translational control mechanisms instills nanos accumulation uniquely in the sMic lineage.
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Registered trials
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