ArticleInfection and immunity2013
The type VI secretion system encoded in Salmonella pathogenicity island 19 is required for Salmonella enterica serotype Gallinarum survival within infected macrophages.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 74 citations in OpenAlex.
- The SPI-20 and SPI-21 T6SS gene clusters fromFrontiers in microbiology · 2026Article
- From past to present: the crosstalk between type VI secretion system of Salmonella and host.Archives of microbiology · 2025Review
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- Genomic and immunocyte characterisation of bloodstream infection caused by Klebsiella pneumoniae.Annals of clinical microbiology and antimicrobials · 2024Article
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- Genomic analysis ofFrontiers in microbiology · 2024Article
- Unraveling the role of type 1 fimbriae in Salmonella pathogenesis: insights from a comparative analysis of Salmonella Enteritidis and Salmonella Gallinarum.Poultry science · 2023Article
- Transfer of T6SSPolish journal of microbiology · 2023Article
- Bacterial type VI secretion system (T6SS): an evolved molecular weapon with diverse functionality.Biotechnology letters · 2023Review
- Identification and distribution of new candidate T6SS effectors encoded inFrontiers in microbiology · 2023Article
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- Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition inFrontiers in microbiology · 2022Article
- Whole-Genome Comparative and Pathogenicity Analysis ofG3 (Bethesda, Md.) · 2020Article
- Bacteriophage-Insensitive Mutants of Antimicrobial-ResistantInternational journal of molecular sciences · 2020Article
- Modulation ofFrontiers in genetics · 2020Article
- Genetic boundaries delineate the potential human pathogen Salmonella bongori into discrete lineages: divergence and speciation.BMC genomics · 2019Article
- Geography Shapes the Population Genomics of Salmonella enterica Dublin.Genome biology and evolution · 2019Article
- Loss and Gain in the Evolution of themSphere · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica serotype Gallinarum is the causative agent of fowl typhoid, a disease characterized by high morbidity and mortality that causes major economic losses in poultry production. We have reported that S. Gallinarum harbors a type VI secretion system (T6SS) encoded in Salmonella pathogenicity island 19 (SPI-19) that is required for efficient colonization of chicks. In the present study, we aimed to characterize the SPI-19 T6SS functionality and to investigate the mechanisms behind the phenotypes previously observed in vivo. Expression analyses revealed that SPI-19 T6SS core components are expressed and produced under in vitro bacterial growth conditions. However, secretion of the structural/secreted components Hcp1, Hcp2, and VgrG to the culture medium could not be determined, suggesting that additional signals are required for T6SS-dependent secretion of these proteins. In vitro bacterial competition assays failed to demonstrate a role for SPI-19 T6SS in interbacterial killing. In contrast, cell culture experiments with murine and avian macrophages (RAW264.7 and HD11, respectively) revealed production of a green fluorescent protein-tagged version of VgrG soon after Salmonella uptake. Furthermore, infection of RAW264.7 and HD11 macrophages with deletion mutants of SPI-19 or strains with genes encoding specific T6SS core components (clpV and vgrG) revealed that SPI-19 T6SS contributes to S. Gallinarum survival within macrophages at 20 h postuptake. SPI-19 T6SS function was not linked to Salmonella-induced cytotoxicity or cell death of infected macrophages, as has been described for other T6SS. Our data indicate that SPI-19 T6SS corresponds to a novel tool used by Salmonella to survive within host cells.
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