ArticleCellular microbiology2013
Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility.
Article in Cellular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Two Plasmodium vivax hypnozoite-expressed RNA-binding proteins inhibit liver stage replication.Nature communications · 2026Article
- Widespread release of translational repression across Plasmodium's host-to-vector transmission event.PLoS pathogens · 2025Article
- Comparative genomics of Plasmodium yoelii nigeriensis N67 and N67C: genome-wide polymorphisms, differential gene expression, and drug resistance.BMC genomics · 2024Article
- Global Release of Translational Repression AcrossbioRxiv : the preprint server for biology · 2024Article
- scCorrector: a robust method for integrating multi-study single-cell data.Briefings in bioinformatics · 2024Article
- Same, same but different: Exploring Plasmodium cell division during liver stage development.PLoS pathogens · 2023Review
- Long-Read Genome Assembly and Gene Model Annotations for the Rodent Malaria ParasitebioRxiv : the preprint server for biology · 2023Article
- Single-cell RNA sequencing of Plasmodium vivax sporozoites reveals stage- and species-specific transcriptomic signatures.PLoS neglected tropical diseases · 2022Article
- Plasmodium sporozoite disintegration during skin passage limits malaria parasite transmission.EMBO reports · 2022Article
- Dietary supplementation withAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- The Plasmodium NOT1-G paralogue is an essential regulator of sexual stage maturation and parasite transmission.PLoS biology · 2021Article
- A systems-level gene regulatory network model for Plasmodium falciparum.Nucleic acids research · 2021Article
- Plasmodium falciparum LipB mutants display altered redox and carbon metabolism in asexual stages and cannot complete sporogony in Anopheles mosquitoes.International journal for parasitology · 2021Article
- Metabolic Survival Adaptations of Plasmodium falciparum Exposed to Sublethal Doses of Fosmidomycin.Antimicrobial agents and chemotherapy · 2021Article
- Pleiotropic Roles for theFrontiers in cellular and infection microbiology · 2021Article
- NcPuf1 Is a Key Virulence Factor inPathogens (Basel, Switzerland) · 2020Article
- RNA Binding Proteins and Gene Expression Regulation inFrontiers in cellular and infection microbiology · 2020Article
- Preparing for Transmission: Gene Regulation inFrontiers in cellular and infection microbiology · 2020Review
- Protein-RNA interactions important for Plasmodium transmission.PLoS pathogens · 2019Review
- Transcriptomics and proteomics reveal two waves of translational repression during the maturation of malaria parasite sporozoites.Nature communications · 2019Article
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7 authors.
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Abstract
Malaria's cycle of infection requires parasite transmission between a mosquito vector and a mammalian host. We here demonstrate that the Plasmodium yoelii Pumilio-FBF family member Puf2 allows the sporozoite to remain infectious in the mosquito salivary glands while awaiting transmission. Puf2 mediates this solely through its RNA-binding domain (RBD) likely by stabilizing or hastening the degradation of specific mRNAs. Puf2 traffics to sporozoite cytosolic granules, which are negative for several markers of stress granules and P-bodies, and disappear rapidly after infection of hepatocytes. In contrast to previously described Plasmodium berghei pbpuf2(-) parasites, pypuf2(-) sporozoites have no apparent defect in host infection when tested early in salivary gland residence, but become progressively non-infectious and prematurely transform into EEFs during prolonged salivary gland residence. The premature overexpression of Puf2 in oocysts causes striking deregulation of sporozoite maturation and infectivity while extension of Puf2 expression in liverstages causes no defect, suggesting that the presence of Puf2 alone is not sufficient for its functions. Finally, by conducting the first comparative RNA-seq analysis of Plasmodium sporozoites, we find that Puf2 may play a role in directly or indirectly maintaining the homeostasis of specific transcripts. These findings uncover requirements for maintaining a window of opportunity for the malaria parasite to accommodate the unpredictable moment of transmission from mosquito to mammalian host.
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