ArticlePloS one2013
In vivo cell reprogramming towards pluripotency by virus-free overexpression of defined factors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Functional rejuvenation of endothelial cell aging by transient reprogramming.Basic research in cardiology · 2026Article
- Epigenome editing technologies for discovery and medicine.Nature biotechnology · 2024Review
- A Youthful Touch: Reversal of Aging Hallmarks by Cell Reprogramming.Cells, tissues, organs · 2024Review
- Hydrodynamic Delivery: Characteristics, Applications, and Technological Advances.Pharmaceutics · 2023Review
- Review
- Article
- Epigenetic changes during aging and their reprogramming potential.Critical reviews in biochemistry and molecular biology · 2019Review
- Non-viral, Tumor-free Induction of Transient Cell Reprogramming in Mouse Skeletal Muscle to Enhance Tissue Regeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Evolving principles underlying neural lineage conversion and their relevance for biomedical translation.F1000Research · 2019Review
- In Vivo Transient and Partial Cell Reprogramming to Pluripotency as a Therapeutic Tool for Neurodegenerative Diseases.Molecular neurobiology · 2018Review
- AAVvector-mediated in vivo reprogramming into pluripotency.Nature communications · 2018Article
- Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System.Stem cells international · 2018Review
- The therapeutic potential of cell identity reprogramming for the treatment of aging-related neurodegenerative disorders.Progress in neurobiology · 2017Review
- Transient transcription factor (OSKM) expression is key towards clinical translation ofEMBO molecular medicine · 2017Article
- Cocktail of chemical compounds robustly promoting cell reprogramming protects liver against acute injury.Protein & cell · 2017Article
- Direct conversion of mouse embryonic fibroblasts into functional keratinocytes through transient expression of pluripotency-related genes.Stem cell research & therapy · 2016Article
- Engineering Cell Fate for Tissue Regeneration by In Vivo Transdifferentiation.Stem cell reviews and reports · 2016Review
- Regeneration through reprogramming adult cell identity in vivo.The American journal of pathology · 2015Review
- Expanding horizons of cellular plasticity in regenerative medicine.The American journal of pathology · 2015Article
- In vivo reprogrammed pluripotent stem cells from teratomas share analogous properties with their in vitro counterparts.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The ability to induce the reprogramming of somatic mammalian cells to a pluripotent state by the forced expression of specific transcription factors has helped redefine the rules of cell fate and plasticity, as well as open possibilities for disease modeling, drug screening and regenerative medicine. Here, we hypothesized that the non-viral forced expression of the four originally discovered defined factors (OKSM) in adult mice could result in in vivo reprogramming of cells in the transfected tissue in situ. We show that a single hydrodynamic tail-vein (HTV) injection of two plasmids encoding for Oct3/4, Sox2, Klf4 and c-Myc respectively, are highly expressed in the liver tissue of Balb/C adult mice. Hallmark pluripotency markers were upregulated within 24-48 h after injection, followed by down-regulation of all major hepatocellular markers. Generation of transcriptionally reprogrammed cells in vivo was further confirmed by positive staining of liver tissue sections for all major pluripotency markers in Balb/C mice and the Nanog-GFP reporter transgenic strain (TNG-A) with concomitant upregulation of GFP expression in situ. No signs of physiological or anatomical abnormalities or teratoma formation were observed in the liver examined up to 120 days. These findings indicate that virus-free expression of OKSM factors in vivo can transcriptionally reprogram cells in situ rapidly, efficiently and transiently, absent of host tissue damage or teratoma formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.