ArticlePlant physiology2013
A robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin signaling network in planta.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 184 papers.
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184 citing papers in PubMed, 356 citations in OpenAlex.
- Quantitative live-cell imaging of auxin and cytokinin signalling in developing feeding sites ofPlant signaling & behavior · 2026Article
- Evolutionary origin of a new gene drives root growth in one desert poplar.The New phytologist · 2026Article
- Selective autophagy receptor VISP1 enhances cytokinin signaling by modulating the Type-A/B ARR feedback loop during root development.Science advances · 2026Article
- Cytokinin receptor AHK3 influences leaf size by modulating trans-zeatin-type cytokinin levels in xylem.Plant & cell physiology · 2026Article
- A single-cell screening platform accelerates functional genetics in plants.Nature biotechnology · 2026Article
- Auxin and cytokinin regulate growth dynamics underlying carpel initiation in Arabidopsis.Journal of experimental botany · 2026Article
- LL3, a homolog of LONESOME HIGHWAY, regulates vascular cell proliferation in the root apical meristem.Plant & cell physiology · 2026Article
- Synthetic promoter design in plants: integration of computational and experimental approaches.Frontiers in plant science · 2026Review
- The trehalose 6-phosphate pathway coordinates dynamic changes at the shoot apical meristem in Arabidopsis thaliana.Plant physiology · 2025Article
- Geminiviral replication enhancer hijacks a negative regulator of cytokinin signaling to facilitate viral infection.Plant communications · 2025Article
- A cytokinin response maximum induces and activates bifacial stem cells for radial growth.Nature plants · 2025Article
- How meristems shape plant architecture in cereals-Cereal Stem Cell Systems (CSCS) Consortium.The Plant cell · 2025Review
- The metabolite itaconate is a transcriptional and posttranslational modulator of plant metabolism, development, and stress response.Science advances · 2025Article
- Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits.The Plant cell · 2025Article
- Confocal imaging of the cell cycle and cytokinin signaling during gynoecium development in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Identification of a Specific Role of Dihydrozeatin in the Regulation of the Cell Differentiation Activity inPlants (Basel, Switzerland) · 2025Article
- Structural basis of cytokinin transport by the Arabidopsis AZG2.Nature communications · 2025Article
- A long-distance inhibitory system regulates haustoria numbers in parasitic plants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Investigating biological nitrogen fixation via single-cell transcriptomics.Journal of experimental botany · 2025Review
- Genome-wide characterization of two-component system elements in barley enables the identification of grain-specific phosphorelay genes.BMC plant biology · 2025Article
124 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins are classic plant hormones that orchestrate plant growth, development, and physiology. They affect gene expression in target cells by activating a multistep phosphorelay network. Type-B response regulators, acting as transcriptional activators, mediate the final step in the signaling cascade. Previously, we have introduced a synthetic reporter, Two Component signaling Sensor (TCS)::green fluorescent protein (GFP), which reflects the transcriptional activity of type-B response regulators. TCS::GFP was instrumental in uncovering roles of cytokinin and deepening our understanding of existing functions. However, TCS-mediated expression of reporters is weak in some developmental contexts where cytokinin signaling has a documented role, such as in the shoot apical meristem or in the vasculature of Arabidopsis (Arabidopsis thaliana). We also observed that GFP expression becomes rapidly silenced in TCS::GFP transgenic plants. Here, we present an improved version of the reporter, TCS new (TCSn), which, compared with TCS, is more sensitive to phosphorelay signaling in Arabidopsis and maize (Zea mays) cellular assays while retaining its specificity. Transgenic Arabidopsis TCSn::GFP plants exhibit strong and dynamic GFP expression patterns consistent with known cytokinin functions. In addition, GFP expression has been stable over generations, allowing for crosses with different genetic backgrounds. Thus, TCSn represents a significant improvement to report the transcriptional output profile of phosphorelay signaling networks in Arabidopsis, maize, and likely other plants that display common response regulator DNA-binding specificities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.