ArticleThe Journal of cell biology1990
Detection of extracellular calcium gradients with a calcium-specific vibrating electrode.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed.
- SISE, free LabView-based software for ion flux measurements.Plant methods · 2025Article
- Non-Invasive Micro-Test Technology in Plant Physiology Under Abiotic Stress: From Mechanism to Application.Plants (Basel, Switzerland) · 2025Review
- Non-invasive micro-test technology and applications.Biophysics reports · 2025Article
- Superior glucose metabolism supports NHFrontiers in plant science · 2024Article
- ATP-mediated increase in HJournal of neurophysiology · 2024Article
- Overexpression of a Plasma Membrane HInternational journal of molecular sciences · 2022Article
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments.Plant physiology · 2022Article
- Extracellular ATP-Induced Alterations in Extracellular HFrontiers in cellular neuroscience · 2021Article
- miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells.Frontiers in cell and developmental biology · 2021Article
- ABA-dependent KPlant signaling & behavior · 2020Article
- Real-time physiological measurements of oxygen using a non-invasive self-referencing optical fiber microsensor.Nature protocols · 2020Article
- The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.International journal of molecular sciences · 2018Review
- Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux.PloS one · 2018Article
- Oscillatory signatures underlie growth regimes in Arabidopsis pollen tubes: computational methods to estimate tip location, periodicity, and synchronization in growing cells.Journal of experimental botany · 2017Article
- The microbe-secreted isopeptide poly-γ-glutamic acid induces stress tolerance in Brassica napus L. seedlings by activating crosstalk between HScientific reports · 2017Article
- Influence of Electric Fields and Conductivity on Pollen Tube Growth assessed via Electrical Lab-on-Chip.Scientific reports · 2016Article
- Recent advances in understanding the extracellular calcium-sensing receptor.F1000Research · 2016Review
- Article
- Measurement of extracellular ion fluxes using the ion-selective self-referencing microelectrode technique.Journal of visualized experiments : JoVE · 2015Article
- Flowers under pressure: ins and outs of turgor regulation in development.Annals of botany · 2014Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a vibrating calcium-specific electrode to measure minute extracellular calcium gradients and thus infer the patterns of calcium currents that cross the surface of various cells and tissues. Low-resistance calcium electrodes (routinely approximately 500 M omega) are vibrated by means of orthogonally stacked piezoelectrical pushers, driven by a damped square wave at an optimal frequency of 0.5 Hz. Phase-sensitive detection of the electrode signal is performed with either analogue or digital electronics. The resulting data are superimposed on a video image of the preparation that is being measured. Depending on the background calcium concentration, this new device can readily and reliably measure steady extracellular differences of calcium concentration which are as small as 0.01% with spatial and temporal resolutions of a few microns and a few seconds, respectively. The digital version can attain a noise level of less than 1 microV. In exploratory studies, we have used this device to map and measure the patterns of calcium currents that cross the surface of growing fucoid eggs and tobacco pollen, moving amebae and Dictyostelium slugs, recently fertilized ascidian eggs, as well as nurse cells of Sarcophaga follicles. This approach should be easily extendable to other specific ion currents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.