ArticleNucleic acids research2013
UV damage regulates alternative polyadenylation of the RPB2 gene in yeast.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.Nucleic acids research · 2020Article
- Connections between 3' end processing and DNA damage response: Ten years later.Wiley interdisciplinary reviews. RNA · 2020Review
- Dysregulation of Alternative Poly-adenylation as a Potential Player in Autism Spectrum Disorder.Frontiers in molecular neuroscience · 2017Article
- The differential expression of alternatively polyadenylated transcripts is a common stress-induced response mechanism that modulates mammalian mRNA expression in a quantitative and qualitative fashion.RNA (New York, N.Y.) · 2016Article
- RNA polymerase II depletion promotes transcription of alternative mRNA species.BMC molecular biology · 2016Article
- Promoter-Terminator Gene Loops Affect Alternative 3'-End Processing in Yeast.The Journal of biological chemistry · 2016Article
- The Sub1 nuclear protein protects DNA from oxidative damage.Molecular and cellular biochemistry · 2016Article
- Intronic cleavage and polyadenylation regulates gene expression during DNA damage response through U1 snRNA.Cell discovery · 2016Article
- Transcription bypass of DNA lesions enhances cell survival but attenuates transcription coupled DNA repair.Nucleic acids research · 2014Article
- Insights into how Spt5 functions in transcription elongation and repressing transcription coupled DNA repair.Nucleic acids research · 2014Article
- The polyadenylation code: a unified model for the regulation of mRNA alternative polyadenylation.Journal of Zhejiang University. Science. B · 2014Review
- DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast.Genome research · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3'UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3'UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.