ArticleThe Journal of biological chemistry2013
Charge composition features of model single-span membrane proteins that determine selection of YidC and SecYEG translocase pathways in Escherichia coli.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- How mutagenesis and laboratory conditions affect the genome of the metal-resistant bacteriumJournal of bacteriology · 2026Article
- The versatile role of YidC in membrane protein biosynthesis and quality control.Biochimica et biophysica acta. Molecular cell research · 2025Review
- Interaction between glycolipid MPIase and proteinaceous factors during protein integration into the cytoplasmic membrane ofFrontiers in molecular biosciences · 2022Review
- Interplay between MPIase, YidC, and PMF during Sec-independent insertion of membrane proteins.Life science alliance · 2022Article
- Pushing the Envelope: The Mysterious Journey Through the Bacterial Secretory Machinery, and Beyond.Frontiers in microbiology · 2021Review
- Sec translocon has an insertase-like function in addition to polypeptide conduction through the channel.F1000Research · 2019Review
- The Conserved Role of YidC in Membrane Protein Biogenesis.Microbiology spectrum · 2019Article
- Targeting and Insertion of Membrane Proteins.EcoSal Plus · 2017Review
- Role of the Cytosolic Loop C2 and the C Terminus of YidC in Ribosome Binding and Insertion Activity.The Journal of biological chemistry · 2015Article
- YidC/Alb3/Oxa1 Family of Insertases.The Journal of biological chemistry · 2015Review
- SecA drives transmembrane insertion of RodZ, an unusual single-span membrane protein.Journal of molecular biology · 2015Article
- MifM monitors total YidC activities of Bacillus subtilis, including that of YidC2, the target of regulation.Journal of bacteriology · 2015Article
- Charge requirements of lipid II flippase activity in Escherichia coli.Journal of bacteriology · 2014Article
- Article
- Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein.The Journal of biological chemistry · 2014Article
- Role of the twin arginine protein transport pathway in the assembly of the Streptomyces coelicolor cytochrome bc1 complex.Journal of bacteriology · 2014Article
- YidC protein, a molecular chaperone for LacY protein folding via the SecYEG protein machinery.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We have investigated the features of single-span model membrane proteins based upon leader peptidase that determines whether the proteins insert by a YidC/Sec-independent, YidC-only, or YidC/Sec mechanism. We find that a protein with a highly hydrophobic transmembrane segment that inserts into the membrane by a YidC/Sec-independent mechanism becomes YidC-dependent if negatively charged residues are inserted into the translocated periplasmic domain or if the hydrophobicity of the transmembrane segment is reduced by substituting polar residues for nonpolar ones. This suggests that charged residues in the translocated domain and the hydrophobicity within the transmembrane segment are important determinants of the insertion pathway. Strikingly, the addition of a positively charged residue to either the translocated region or the transmembrane region can switch the insertion requirements such that insertion requires both YidC and SecYEG. To test conclusions from the model protein studies, we confirmed that a positively charged residue is a SecYEG determinant for the endogenous proteins ATP synthase subunits a and b and the TatC subunit of the Tat translocase. These findings provide deeper insights into how pathways are selected for the insertion of proteins into the Escherichia coli inner membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.