ArticleJournal of bacteriology2013
AmrZ modulates Pseudomonas aeruginosa biofilm architecture by directly repressing transcription of the psl operon.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
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- Broad Host Range Peptide Nucleic Acids Prevent Gram-Negative Biofilms Implicated in Catheter-Associated Urinary Tract Infections.Microorganisms · 2025Article
- Co-culture biofilm patterns among different Pseudomonas aeruginosa clones from cystic fibrosis patients.Biofilm · 2025Article
- SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa.NPJ biofilms and microbiomes · 2025Article
- Extracellular aminopeptidase regulates exopolysaccharide production of Pseudomonas aeruginosa via quorum sensing.The ISME journal · 2025Article
- Oligonucleotide library assisted sequence mining reveals promoter sequences with distinct temporal expression dynamics for applications inSynthetic biology (Oxford, England) · 2025Article
- Flip the switch: the role of FleQ in modulating the transition between the free-living and sessile mode of growth inJournal of bacteriology · 2024Review
- Clinical characteristics, risk factors, and outcomes of patients with polymicrobial Pseudomonas aeruginosa bloodstream infections.Journal of the Chinese Medical Association : JCMA · 2023Observational
- Adaption ofMicroorganisms · 2023Review
- Type II Toxin-Antitoxin Systems inToxins · 2023Review
- AIDmut-Seq: a Three-Step Method for Detecting Protein-DNA Binding Specificity.Microbiology spectrum · 2023Article
- The Alginate and Motility Regulator AmrZ is Essential for the Regulation of the Dispersion Response bymSphere · 2022Article
- Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP.Veterinary research · 2022Article
- Detoxification Response ofMicroorganisms · 2022Article
- Regulation of extracellular matrix components by AmrZ is mediated by c-di-GMP in Pseudomonas ogarae F113.Scientific reports · 2022Article
- PrrT/A, a Pseudomonas aeruginosa Bacterial Encoded Toxin-Antitoxin System Involved in Prophage Regulation and Biofilm Formation.Microbiology spectrum · 2022Article
- Interbacterial Antagonism Mediated by a Released Polysaccharide.Journal of bacteriology · 2022Article
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4 authors at 3 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa strains recovered from chronic pulmonary infections in cystic fibrosis patients are frequently mucoid. Such strains express elevated levels of alginate but reduced levels of the aggregative polysaccharide Psl; however, the mechanistic basis for this regulation is not completely understood. Elevated pslA expression was observed in an amrZ null mutant and in strains expressing a DNA-binding-deficient AmrZ. AmrZ is a transcription factor that positively regulates twitching motility and alginate synthesis, two phenotypes involved in P. aeruginosa biofilm development. AmrZ bound directly to the pslA promoter in vitro, and molecular analyses indicate that AmrZ represses psl expression by binding to a site overlapping the promoter. Altered expression of amrZ in nonmucoid strains impacted biofilm structure and architecture, as structured microcolonies were observed with low AmrZ production and flat biofilms with amrZ overexpression. These biofilm phenotypes correlated with Psl levels, since we observed elevated Psl production in amrZ mutants and lower Psl production in amrZ-overexpressing strains. These observations support the hypothesis that AmrZ is a multifunctional regulator mediating transition of P. aeruginosa biofilm infections from colonizing to chronic biofilms through repression of the psl operon while activating the algD operon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.