ArticleJournal of bacteriology2013
Regulation, integrase-dependent excision, and horizontal transfer of genomic islands in Legionella pneumophila.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.Current opinion in microbiology · 2024Review
- Bioinformatic and experimental characterization of SEN1998: a conserved gene carried by the Enterobacteriaceae-associated ROD21-like family of genomic islands.Scientific reports · 2022Article
- Review
- Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection.PLoS pathogens · 2019Article
- Diverse conjugative elements silence natural transformation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Legionella pneumophila p45 element influences host cell entry and sensitivity to sodium.PloS one · 2019Article
- Diversity-generating retroelements: natural variation, classification and evolution inferred from a large-scale genomic survey.Nucleic acids research · 2018Article
- Construction of a New Phage Integration Vector pFIV-Val for Use in DifferentFrontiers in cellular and infection microbiology · 2018Article
- Review
- Unusual Legionnaires' outbreak in cool, dry Western Canada: an investigation using genomic epidemiology.Epidemiology and infection · 2017Article
- Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila.The Journal of biological chemistry · 2016Article
- csrT Represents a New Class of csrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila.Journal of bacteriology · 2016Article
- Article
- Comparative analyses of Legionella species identifies genetic features of strains causing Legionnaires' disease.Genome biology · 2014Article
- Surface display of a massively variable lipoprotein by a Legionella diversity-generating retroelement.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A distinct and divergent lineage of genomic island-associated Type IV Secretion Systems in Legionella.PloS one · 2013Article
- Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella pneumophila is a Gram-negative freshwater agent which multiplies in specialized nutrient-rich vacuoles of amoebae. When replicating in human alveolar macrophages, Legionella can cause Legionnaires' disease. Recently, we identified a new type of conjugation/type IVA secretion system (T4ASS) in L. pneumophila Corby (named trb-tra). Analogous versions of trb-tra are localized on the genomic islands Trb-1 and Trb-2. Both can exist as an episomal circular form, and Trb-1 can be transferred horizontally to other Legionella strains by conjugation. In our current work, we discovered the importance of a site-specific integrase (Int-1, lpc2818) for the excision and conjugation process of Trb-1. Furthermore, we identified the genes lvrRABC (lpc2813 to lpc2816) to be involved in the regulation of Trb-1 excision. In addition, we demonstrated for the first time that a Legionella genomic island (LGI) of L. pneumophila Corby (LpcGI-2) encodes a functional type IV secretion system. The island can be transferred horizontally by conjugation and is integrated site specifically into the genome of the transconjugants. LpcGI-2 generates three different episomal forms. The predominant episomal form, form A, is generated integrase dependently (Lpc1833) and transferred by conjugation in a pilT-dependent manner. Therefore, the genomic islands Trb-1 and LpcGI-2 should be classified as integrative and conjugative elements (ICEs). Coculture studies of L. pneumophila wild-type and mutant strains revealed that the int-1 and lvrRABC genes (located on Trb-1) as well as lpc1833 and pilT (located on LpcGI-2) do not influence the in vivo fitness of L. pneumophila in Acanthamoeba castellanii.
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