ArticleInternational journal of molecular sciences2013
Efficient Agrobacterium-Mediated Transformation of Hybrid Poplar Populus davidiana Dode x Populus bollena Lauche.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Exploration of Suitable Conditions for Shoot Proliferation and Rooting ofLife (Basel, Switzerland) · 2025Article
- Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar.Life (Basel, Switzerland) · 2023Article
- Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method.Plant methods · 2023Article
- Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance inFrontiers in plant science · 2021Article
- EfficientInternational journal of molecular sciences · 2019Article
- Overexpression ofGenes · 2018Article
- Small GTP-binding protein PdRanBP regulates vascular tissue development in poplar.BMC genetics · 2016Article
- Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera.Frontiers in plant science · 2016Article
- An efficient Agrobacterium-mediated transformation system for poplar.International journal of molecular sciences · 2014Article
- Transgenic hybrid poplar for sustainable and scalable production of the commodity/specialty chemical, 2-phenylethanol.PloS one · 2013Article
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5 authors.
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Abstract
Poplar is a model organism for high in vitro regeneration in woody plants. We have chosen a hybrid poplar Populus davidiana Dode x Populus bollena Lauche. By optimizing the Murashige and Skoog medium with (0.3 mg/L) 6-benzylaminopurine and (0.08 mg/L) naphthaleneacetic acid, we have achieved the highest frequency (90%) for shoot regeneration from poplar leaves. It was also important to improve the transformation efficiency of poplar for genetic breeding and other applications. In this study, we found a significant improvement of the transformation frequency by controlling the leaf age. Transformation efficiency was enhanced by optimizing the Agrobacterium concentration (OD(600) = 0.8-1.0) and an infection time (20-30 min). According to transmission electron microscopy observations, there were more Agrobacterium invasions in the 30-day-old leaf explants than in 60-day-old and 90-day-old explants. Using the green fluorescent protein (GFP) marker, the expression of MD-GFP fusion proteins in the leaf, shoot, and root of hybrid poplar P. davidiana Dode x Populus. bollena Lauche was visualized for confirmation of transgene integration. Southern and Northern blot analysis also showed the integration of T-DNA into the genome and gene expression of transgenic plants. Our results suggest that younger leaves had higher transformation efficiency (~30%) than older leaves (10%).
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