ArticleBMC cell biology2013
Collagen/β(1) integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells.
Article in BMC cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- uPAR deficiency triggers TGFβ1-mediated fibrotic remodeling in a cardiac perivascular-like microenvironment.Stem cell research & therapy · 2026Article
- The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres.International journal of molecular sciences · 2025Article
- Dynamic proteome profiling of differentiating human embryonic stem cells towards cardiomyocytes.Scientific reports · 2025Article
- Article
- Nanohydroxyapatite-Coated Titanium Surface Increases Vascular Endothelial Cells Distinct Signaling Responding to High Glucose Concentration.Journal of functional biomaterials · 2023Article
- Effect of Rho-Associated Kinase Inhibitor on Growth Behaviors of Human Induced Pluripotent Stem Cells in Suspension Culture.Bioengineering (Basel, Switzerland) · 2022Article
- CCBE1 in Cardiac Development and Disease.Frontiers in genetics · 2022Review
- Three-Dimensional Poly-(ε-Caprolactone) Nanofibrous Scaffolds Promote the Maturation of Human Pluripotent Stem Cells-Induced Cardiomyocytes.Frontiers in cell and developmental biology · 2022Article
- Assessment of the In Vitro Cytotoxicity Effects of the Leaf Methanol Extract ofPharmaceuticals (Basel, Switzerland) · 2021Article
- The vascular niche in next generation microphysiological systems.Lab on a chip · 2021Review
- Conductive single-wall carbon nanotubes/extracellular matrix hybrid hydrogels promote the lineage-specific development of seeding cells for tissue repair through reconstructing an integrin-dependent niche.Journal of nanobiotechnology · 2021Article
- Building an Artificial Cardiac Microenvironment: A Focus on the Extracellular Matrix.Frontiers in cell and developmental biology · 2020Review
- Effect of liquid flow by pipetting during medium change on deformation of hiPSC aggregates.Regenerative therapy · 2019Article
- Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.Stem cells international · 2019Review
- Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells.PloS one · 2018Article
- Role of alpha- and beta-adrenergic receptors in cardiomyocyte differentiation from murine-induced pluripotent stem cells.Cell proliferation · 2017Article
- Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics.Stem cells international · 2017Review
- Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling.BMC developmental biology · 2016Article
- A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Mechanical Forces Reshape Differentiation Cues That Guide Cardiomyogenesis.Circulation research · 2016Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe interactions between stem cells and extracellular matrix (ECM) mediated by integrins play important roles in the processes that determine stem cell fate. However, the role of ECM/integrin interaction in the formation of embryoid bodies (EBs) during cardiogenesis from murine induced pluripotent stem cells (miPSCs) remains unclear.
resultsIn the present study, collagen type I and β(1) integrin were expressed and upregulated synergistically during the formation of miPSC-derived EBs, with a peak expression at day 3 of differentiation. The blockage of collagen/β(1) integrin interaction by β(1) integrin blocking antibody resulted in the production of defective EBs that were characterized by decreased size and the absence of a shell-like layer composed of primitive endoderm cells. The quantification of spontaneous beating activity, cardiac-specific gene expression and cardiac troponin T (cTnT) immunostaining showed that the cardiac differentiation of these defective miPSC-derived EBs was lower than that of control EBs.
conclusionsThese findings indicate that collagen/β(1) integrin interaction is required for the growth and cardiac differentiation of miPSC-derived EBs and will be helpful in future engineering of the matrix microenvironment within EBs to efficiently direct the cardiac fate of pluripotent stem cells to promote cardiovascular regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.