ArticlePLoS neglected tropical diseases2013
A multiplex PCR for the simultaneous detection and genotyping of the Echinococcus granulosus complex.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Occurrence ofVeterinary medicine international · 2026Article
- First comparative proteomic andFrontiers in veterinary science · 2025Article
- Cystic Echinococcosis in the Early 2020s: A Review.Tropical medicine and infectious disease · 2024Review
- G1 the commonJournal of veterinary science · 2024Article
- Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.PLoS neglected tropical diseases · 2023Article
- Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences.Parasitology research · 2023Article
- Occurrence and genetic characterization of Echinococcus granulosus sensu lato from domestic animals in Central Iran.BMC veterinary research · 2022Article
- LAMP Assay for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Species Detection within thePathogens (Basel, Switzerland) · 2020Article
- Targeted Sequencing of Genomic Repeat Regions Detects Circulating Cell-free Echinococcus DNA.PLoS neglected tropical diseases · 2020Article
- Genetic survey of cystic echinococcosis in farm animals in Oman.Tropical animal health and production · 2020Article
- Genotyping of humanJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2019Article
- A multiplex PCR for differential detection of Echinococcus granulosus sensu stricto, Echinococcus multilocularis and Echinococcus canadensis in China.Infectious diseases of poverty · 2019Article
- Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1-G3 genotype.Molecular biology reports · 2019Article
- Genetic Diversity of Echinococcus granulosus Genotype G1 in Xinjiang, Northwest of China.The Korean journal of parasitology · 2018Article
- Practical Algorisms for PCR-RFLP-Based Genotyping of Echinococcus granulosus Sensu Lato.The Korean journal of parasitology · 2017Article
- Is there echinococcosis in West Africa? A refugee from Niger with a liver cyst.Parasites & vectors · 2017Article
- Human cystic echinococcosis in Morocco: Ultrasound screening in the Mid Atlas through an Italian-Moroccan partnership.PLoS neglected tropical diseases · 2017Article
- Molecular characterization of Echinococcus granulosus isolates from Bulgarian human cystic echinococcosis patients.Parasitology research · 2017Article
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11 authors.
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Abstract
Echinococcus granulosus is characterized by high intra-specific variability (genotypes G1-G10) and according to the new molecular phylogeny of the genus Echinococcus, the E. granulosus complex has been divided into E. granulosus sensu stricto (G1-G3), E. equinus (G4), E. ortleppi (G5), and E. canadensis (G6-G10). The molecular characterization of E. granulosus isolates is fundamental to understand the spatio-temporal epidemiology of this complex in many endemic areas with the simultaneous occurrence of different Echinococcus species and genotypes. To simplify the genotyping of the E. granulosus complex we developed a single-tube multiplex PCR (mPCR) allowing three levels of discrimination: (i) Echinococcus genus, (ii) E. granulosus complex in common, and (iii) the specific genotype within the E. granulosus complex. The methodology was established with known DNA samples of the different strains/genotypes, confirmed on 42 already genotyped samples (Spain: 22 and Bulgaria: 20) and then successfully applied on 153 unknown samples (Tunisia: 114, Algeria: 26 and Argentina: 13). The sensitivity threshold of the mPCR was found to be 5 ng Echinoccoccus DNA in a mixture of up to 1 µg of foreign DNA and the specificity was 100% when template DNA from closely related members of the genus Taenia was used. Additionally to DNA samples, the mPCR can be carried out directly on boiled hydatid fluid or on alkaline-lysed frozen or fixed protoscoleces, thus avoiding classical DNA extractions. However, when using Echinococcus eggs obtained from fecal samples of infected dogs, the sensitivity of the mPCR was low (<40%). Thus, except for copro analysis, the mPCR described here has a high potential for a worldwide application in large-scale molecular epidemiological studies on the Echinococcus genus.
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