ArticlePloS one2013
STED nanoscopy with time-gated detection: theoretical and experimental aspects.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- PSF-Driven Spatiotemporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.ACS photonics · 2026Article
- Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.Biomedical optics express · 2026Article
- Molecular mechanism of GPCR spatial organization at the plasma membrane.Nature chemical biology · 2024Article
- Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy.International journal of molecular sciences · 2023Review
- Advanced Microscopy Techniques for Molecular Biophysics.International journal of molecular sciences · 2023Review
- Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis.Nanoscale · 2023Article
- Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli.Current genetics · 2023Article
- Article
- Focus image scanning microscopy for sharp and gentle super-resolved microscopy.Nature communications · 2022Article
- Stimulated emission depletion microscopy with a single depletion laser using five fluorochromes and fluorescence lifetime phasor separation.Scientific reports · 2022Article
- Ultralow Laser Power Three-Dimensional Superresolution Microscopy Based on Digitally Enhanced STED.Biosensors · 2022Article
- Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging.Biosensors · 2021Article
- isoSTED microscopy with water-immersion lenses and background reduction.Biophysical journal · 2021Article
- Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique.The Journal of biological chemistry · 2021Review
- Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens.Methods (San Diego, Calif.) · 2020Review
- Efficient two-photon excitation stimulated emission depletion nanoscope exploiting spatiotemporal information.Neurophotonics · 2019Article
- A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses.Biomedical optics express · 2019Article
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7 authors.
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Abstract
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy using time-gated detection, pulsed excitation and continuous wave (CW) STED beams. We present a theoretical framework and experimental data that characterize the time evolution of the effective point-spread-function of a STED microscope and illustrate the physical basis, the benefits, and the limitations of time-gated detection both for CW and pulsed STED lasers. While gating hardly improves the effective resolution in the all-pulsed modality, in the CW-STED modality gating strongly suppresses low spatial frequencies in the image. Gated CW-STED nanoscopy is in essence limited (only) by the reduction of the signal that is associated with gating. Time-gated detection also reduces/suppresses the influence of local variations of the fluorescence lifetime on STED microscopy resolution.
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