ArticlePloS one2013
Wheat chloroplast targeted sHSP26 promoter confers heat and abiotic stress inducible expression in transgenic Arabidopsis Plants.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 64 citations in OpenAlex.
- Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem-preferred transgene expression.Frontiers in plant science · 2025Article
- ZmHsp18 screened from the ZmHsp20 gene family confers thermotolerance in maize.BMC plant biology · 2024Article
- Genome-wide identification and characterization of NAC transcription factor family members in Trifolium pratense and expression analysis under lead stress.BMC genomics · 2024Article
- Characterization of wheat Wrab18 gene promoter and expression analysis under abiotic stress.Molecular biology reports · 2023Article
- Article
- Identification of candidate regulators of the response to early heat stress in climate-adapted wheat landracesFrontiers in plant science · 2023Article
- Targeted single-cell gene induction by optimizing the dually regulated CRE/Frontiers in plant science · 2023Article
- Drought Stress Pre-Treatment Triggers Thermotolerance Acquisition in Durum Wheat.International journal of molecular sciences · 2022Article
- Article
- Overexpression ofCells · 2022Article
- The Chloroplastic Small Heat Shock Protein GeneInternational journal of genomics · 2021Article
- A heat-shock 20 protein isolated from watermelon (ClHSP22.8) negatively regulates the response ofPeerJ · 2021Article
- TheFrontiers in plant science · 2020Article
- Article
- Dissecting the Molecular Function ofFrontiers in genetics · 2020Article
- The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat.BMC plant biology · 2019Article
- Genome-Wide Analysis of Watermelon HSP20s and Their Expression Profiles and Subcellular Locations under Stresses.International journal of molecular sciences · 2018Article
- Heat in Wheat: Exploit Reverse Genetic Techniques to Discover New Alleles Within theFrontiers in plant science · 2018Article
- The role of Arabidopsis aldehyde dehydrogenase genes in response to high temperature and stress combinations.Journal of experimental botany · 2017Article
- Characterization of 5'UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation.Cell stress & chaperones · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The small heat shock proteins (sHSPs) have been found to play a critical role in physiological stress conditions in protecting proteins from irreversible aggregation. To characterize the hloroplast targeted sHSP26 promoter in detail, deletion analysis of the promoter is carried out and analysed via transgenics in Arabidopsis. In the present study, complete assessment of the importance of CCAAT-box elements along with Heat shock elements (HSEs) in the promoter of sHSP26 was performed. Moreover, the importance of 5' untranslated region (UTR) has also been established in the promoter via Arabidopsis transgenics. An intense GUS expression was observed after heat stress in the transgenics harbouring a full-length promoter, confirming the heat-stress inducibility of the promoter. Transgenic plants without UTR showed reduced GUS expression when compared to transgenic plants with UTR as was confirmed at the RNA and protein levels by qRT-PCR and GUS histochemical assays, thus suggesting the possible involvement of some regulatory elements present in the UTR in heat-stress inducibility of the promoter. Promoter activity was also checked under different abiotic stresses and revealed differential expression in different deletion constructs. Promoter analysis based on histochemical assay, real-time qPCR and fluorimetric analysis revealed that HSEs alone could not transcribe GUS gene significantly in sHSP26 promoter and CCAAT box elements contribute synergistically to the transcription. Our results also provide insight into the importance of 5`UTR of sHsp26 promoter thus emphasizing the probable role of imperfect CCAAT-box element or some novel cis-element with respect to heat stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.