ArticlePloS one2013
Characterization of adult α- and β-globin elevated by hydrogen peroxide in cervical cancer cells that play a cytoprotective role against oxidative insults.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 61 citations in OpenAlex.
- Research Progress on Hemoglobin Expression in Non-Erythroid Cells and Tumor Cells.Journal of cellular physiology · 2026Review
- Investigating the Cellular Responses to Combined Nisin and Urolithin B Treatment (7:3) in HKB-11 Lymphoma Cells.International journal of molecular sciences · 2025Article
- Lactate metabolism-related genes serve as potential biomarkers for predicting gastric cancer progression and immunotherapy.Discover oncology · 2025Article
- Hemoglobin alpha is a redox-sensitive mitochondrial-related protein in T-lymphocytes.Free radical biology & medicine · 2025Article
- Hemoglobin alpha is a redox-sensitive mitochondrial-related protein in T-lymphocytes.bioRxiv : the preprint server for biology · 2024Article
- Exploring unconventional attributes of red blood cells and their potential applications in biomedicine.Protein & cell · 2024Article
- Targeting lncRNA16 by GalNAc-siRNA conjugates facilitates chemotherapeutic sensibilization via the HBB/NDUFAF5/ROS pathway.Science China. Life sciences · 2024Article
- A Curcumin-Based Oral Gel Has Potential Protective Efficacy against Oral Mucositis: In Vitro Study.Journal of personalized medicine · 2023Article
- Hemoglobin Subunit Theta 1 Promotes Proliferation by Reducing Reactive Oxygen Species in Lung Adenocarcinoma.Cancers · 2023Article
- Phospho-DIGE Identified Phosphoproteins Involved in Pathways Related to Tumour Growth in Endometrial Cancer.International journal of molecular sciences · 2023Article
- Article
- Hemoglobin β Expression Is Associated with Poor Prognosis in Clear Cell Renal Cell Carcinoma.Biomedicines · 2023Article
- Proteomics profiling for the global and acetylated proteins of papillary thyroid cancers.Proteome science · 2023Article
- 3-Mercaptopyruvate sulfur transferase is a protein persulfidase.Nature chemical biology · 2023Article
- Reprogramming anchorage dependency by adherent-to-suspension transition promotes metastatic dissemination.Molecular cancer · 2023Article
- Effect of the dietary supplementation with extracts of chestnut wood and grape pomace on performance and jejunum response in female and male broiler chickens at different ages.Journal of animal science and biotechnology · 2022Article
- The role of globins in cardiovascular physiology.Physiological reviews · 2022Review
- Proteomics changes after negative pressure wound therapy in diabetic foot ulcers.Molecular medicine reports · 2021Article
- Transcriptome profiles of stem-like cells from primary breast cancers allow identification of ITGA7 as a predictive marker of chemotherapy response.British journal of cancer · 2021Article
- Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium.Scientific reports · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesHemoglobin (Hgb) is the main oxygen and carbon dioxide carrier in cells of erythroid lineage and is responsible for oxygen delivery to the respiring tissues of the body. However, Hgb is also expressed in nonerythroid cells. In the present study, the expression of Hgb in human uterine cervix carcinoma cells and its role in cervical cancer were investigated. METHODOLOGY: The expression level of Hgb in cervical cancer tissues was assessed by quantitative reverse transcriptase-PCR (qRT-PCR). We applied multiple methods, such as RT-PCR, immunoblotting, and immunohistochemical analysis, to confirm Hgb expression in cervical cancer cells. The effects of ectopic expression of Hgb and Hgb mutants on oxidative stress and cell viability were investigated by cellular reactive oxygen species (ROS) analysis and lactate dehydrogenase (LDH) array, respectively. Both Annexin V staining assay by flow cytometry and caspase-3 activity assay were used, respectively, to evaluate cell apoptosis.
resultsqRT-PCR analysis showed that Hgb-α- (HBA1) and Hgb-β-globin (HBB) gene expression was significantly higher in cervical carcinoma than in normal cervical tissues, whereas the expression of hematopoietic transcription factors and erythrocyte specific marker genes was not increased. Immunostaining experiments confirmed the expression of Hgb in cancer cells of the uterine cervix. Hgb mRNA and protein were also detected in the human cervical carcinoma cell lines SiHa and CaSki, and Hgb expression was up-regulated by hydrogen peroxide-induced oxidative stress. Importantly, ectopic expression of wild type HBA1/HBB or HBA1, rather than mutants HBA1(H88R)/HBB(H93R) unable to bind hemo, suppressed oxidative stress and improved cell viability.
conclusionsThe present findings show for the first time that Hgb is expressed in cervical carcinoma cells and may act as an antioxidant, attenuating oxidative stress-induced damage in cervical cancer cells. These data provide a significant impact not only in globin biology but also in understanding of cervical cancer pathogenesis associated with oxidative stress.
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