ArticlePloS one2013
Physiological and cellular responses caused by RNAi- mediated suppression of Snf7 orthologue in western corn rootworm (Diabrotica virgifera virgifera) larvae.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 114 citations in OpenAlex.
- Targeted disruption of vacuolar-sorting protein and inhibitor of apoptosis genes via virus-induced gene silencing alters survival and reproduction of Diaphorina citri.Pest management science · 2026Article
- EndophyticJournal of fungi (Basel, Switzerland) · 2026Article
- Plant resistance: scientific basis and latest research progress.Frontiers in plant science · 2026Review
- Environmental factors affecting RNAi efficacy: Temperature but not plant cultivar influences Colorado potato beetle's response to insecticidal dsRNA.Insect molecular biology · 2025Article
- DsRNA as pathogen-associated molecular pattern in innate immunity and multiple functions of the RNAi machinery complicate the use of RNAi in pest control.Frontiers in insect science · 2025Review
- Biopesticides: a Green Approach Towards Agricultural Pests.Applied biochemistry and biotechnology · 2024Review
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
- Characterization of Ionotropic Receptor GenePlants (Basel, Switzerland) · 2023Article
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- Gene silencing for invasive paper wasp management: Synthesized dsRNA can modify gene expression but did not affect mortality.PloS one · 2023Article
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- Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application.International journal of molecular sciences · 2022Review
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Assessment of genetically modified maize DP4114 × MON 89034 × MON 87411 × DAS-40278-9 and subcombinations, for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA GMO-NL-2020-171).EFSA journal. European Food Safety Authority · 2022Article
- Efficient control of western flower thrips by plastid-mediated RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions.Insects · 2022Review
- Three strategies of transgenic manipulation for crop improvement.Frontiers in plant science · 2022Review
- Article
- First Evidence of Feeding-Induced RNAi in Banana Weevil via Exogenous Application of dsRNA.Insects · 2021Article
- Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA.RNA biology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ingestion of double stranded RNA (dsRNA) has been previously demonstrated to be effective in triggering RNA interference (RNAi) in western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte), providing potential novel opportunities for insect pest control. The putative Snf7 homolog of WCR (DvSnf7) has previously been shown to be an effective RNAi target for insect control, as DvSnf7 RNAi leads to lethality of WCR larvae. Snf7 functions as a part of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway which plays a crucial role in cellular housekeeping by internalization, transport, sorting and lysosomal degradation of transmembrane proteins. To understand the effects that lead to death of WCR larvae by DvSnf7 RNAi, we examined some of the distinct cellular processes associated with ESCRT functions such as de-ubiquitination of proteins and autophagy. Our data indicate that ubiquitinated proteins accumulate in DvSnf7 dsRNA-fed larval tissues and that the autophagy process seems to be impaired. These findings suggest that the malfunctioning of these cellular processes in both midgut and fat body tissues triggered by DvSnf7 RNAi were the main effects leading to the death of WCR. This study also illustrates that Snf7 is an essential gene in WCR and its functions are consistent with biological functions described for other eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.