ArticlePloS one2013
Rapid two-temperature formalin fixation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Impact of Histological Processing on Endogenous Elemental Contents and Distributions in Breast Cancer Tissue Sections.Chemical & biomedical imaging · 2026Article
- AnViruses · 2025Article
- The use of technical grade chemicals and on-site production of ammonium nitrate: a cost-effective and safer approach to Thiel embalming.Surgical and radiologic anatomy : SRA · 2025Article
- Overlap of Formalin-Fixed Paraffin-Embedded and Fresh-Frozen Matched Tissues for Proteomics and Phosphoproteomics.ACS omega · 2025Article
- Be bold, start cold! cold formalin fixation of colorectal cancer specimens granted superior DNA and RNA quality for downstream molecular analysis.Histochemistry and cell biology · 2024Article
- Impact of formalin fixation on mismatch repair protein evaluation by immunohistochemistry.Virchows Archiv : an international journal of pathology · 2023Article
- Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Assessing Tissue Fixation Time and Quality with Label-free Mid Infrared Spectroscopy and Machine Learning.Biopreservation and biobanking · 2023Article
- Antibacterial and Anti-Inflammatory Activities ofMicroorganisms · 2022Article
- Reproducibility of Chronic Changes on High-Quality Deceased Donor Kidney Allograft Biopsies.Kidney international reports · 2022Article
- Making a science out of preanalytics: An analytical method to determine optimal tissue fixation in real-time.PloS one · 2021Article
- Aptamers and Aptamer-Coupled Biosensors to Detect Water-Borne Pathogens.Frontiers in microbiology · 2021Review
- Rapid tissue processing using a temperature-controlled collection device to preserve tumor biomarkers.Cell and tissue banking · 2020Article
- Essentials for Pathological Evaluation of Peritoneal Surface Malignancies and Synoptic Reporting of Cytoreductive Surgery Specimens-A review and evidence-based guide.Indian journal of surgical oncology · 2020Review
- Cold Formalin Fixation Guarantees DNA Integrity in Formalin Fixed Paraffin Embedded Tissues: Premises for a Better Quality of Diagnostic and Experimental Pathology With a Specific Impact on Breast Cancer.Frontiers in oncology · 2020Article
- Monitoring Dehydration and Clearing in Tissue Processing for High-Quality Clinical Pathology.Biopreservation and biobanking · 2019Article
- Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues.Emerging infectious diseases · 2019Article
- Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System.IEEE journal of translational engineering in health and medicine · 2019Article
- Procurement Biopsy Findings Versus Kidney Donor Risk Index for Predicting Renal Allograft Survival.Transplantation direct · 2018Article
- A New Paradigm for Tissue Diagnostics: Tools and Techniques to Standardize Tissue Collection, Transport, and Fixation.Current pathobiology reports · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Formalin fixation is a mainstay of modern histopathologic analysis, yet the practice is poorly standardized and a significant potential source of preanalytical errors. Concerns of workflow and turnaround time drive interest in developing shorter fixation protocols, but rapid protocols can lead to poor histomorphology or inadequate downstream assay results. Additionally, assays such as immunohistochemistry for phosphorylated epitopes have historically been challenging in the context of formalin-fixed tissue, indicating that there may be room for improvement in this process that is fundamental to the practice of anatomic pathology. With these issues in mind, we studied basic formalin biochemistry to develop a novel formalin fixation protocol that involves a pre-incubation in subambient temperature formalin prior to a brief exposure to heated formalin. This new protocol is more rapid than standard protocols yet preserves histomorphology and yields tissue that is compatible with an expanded set of downstream clinical and research assays, including immunohistochemistry for phosphorylated epitopes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.