ArticlePloS one2013
Optimising homing endonuclease gene drive performance in a semi-refractory species: the Drosophila melanogaster experience.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 63 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- CRISPR/Cas9-based split homing gene drive targetingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A CRISPR endonuclease gene drive reveals distinct mechanisms of inheritance bias.Nature communications · 2022Article
- Active genetics comes alive: Exploring the broad applications of CRISPR-based selfish genetic elements (or gene-drives): Exploring the broad applications of CRISPR-based selfish genetic elements (or gene-drives).BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Review
- Protein undernutrition reduces the efficacy of praziquantel in a murine model of Schistosoma mansoni infection.PLoS neglected tropical diseases · 2022Article
- Reflection on the Challenges, Accomplishments, and New Frontiers of Gene Drives.Biodesign research · 2022Review
- Biotechnological Road Map for Innovative Weed Management.Frontiers in plant science · 2022Review
- The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.Frontiers in bioengineering and biotechnology · 2022Review
- Gene drives gaining speed.Nature reviews. Genetics · 2022Review
- Intronic gRNAs for the Construction of Minimal Gene Drive Systems.Frontiers in bioengineering and biotechnology · 2022Article
- Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Integral gene drives for population replacement.Biology open · 2019Article
- Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Consequences of resistance evolution in a Cas9-based sex conversion-suppression gene drive for insect pest management.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonuclease gene (HEG) drive is a promising insect population control technique that employs meganucleases to impair the fitness of pest populations. Our previous studies showed that HEG drive was more difficult to achieve in Drosophila melanogaster than Anopheles gambiae and we therefore investigated ways of improving homing performance in Drosophila. We show that homing in Drosophila responds to increased expression of HEGs specifically during the spermatogonia stage and this could be achieved through improved construct design. We found that 3'-UTR choice was important to maximise expression levels, with HEG activity increasing as we employed Hsp70, SV40, vasa and βTub56D derived UTRs. We also searched for spermatogonium-specific promoters and found that the Rcd-1r promoter was able to drive specific expression at this stage. Since Rcd-1 is a regulator of differentiation in other species, it suggests that Rcd-1r may serve a similar role during spermatogonial differentiation in Drosophila. Contrary to expectations, a fragment containing the entire region between the TBPH gene and the bgcn translational start drove strong HEG expression only during late spermatogenesis rather than in the germline stem cells and spermatogonia as expected. We also observed that the fraction of targets undergoing homing was temperature-sensitive, falling nearly four-fold when the temperature was lowered to 18°C. Taken together, this study demonstrates how a few simple measures can lead to substantial improvements in the HEG-based gene drive strategy and reinforce the idea that the HEG approach may be widely applicable to a variety of insect control programs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.