Evidence map›Paper›PMID 23349776›Full record

ArticlePloS one2013

Self-renewal and differentiation capacity of urine-derived stem cells after urine preservation for 24 hours.

Ren Lang, Guihua Liu, Yingai Shi, Shantaram Bharadwaj, Xiaoyan Leng, Xiaobo Zhou, Hong Liu, Anthony Atala, Yuanyuan Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Tissue engineering of urethra: Systematic review of recent literature.Experimental biology and medicine (Maywood, N.J.) · 2017
    Pooled it
  2. Pooled it
  3. Review
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  5. Article
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  11. Transforming meat waste into sustainable corneal keratoplasty models.Frontiers in bioengineering and biotechnology · 2025
    Article
  12. Review
  13. Challenges of using tissue engineering methods in the treatment of hypospadias.Frontiers in bioengineering and biotechnology · 2025
    Review
  14. Review
  15. Article
  16. Review
  17. Review
  18. Article
  19. Article
  20. Article

23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ren LangDepartment of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, People's Republic of China.
Guihua Liu
Yingai Shi
Shantaram Bharadwaj
Xiaoyan Leng
Xiaobo Zhou
Hong Liu
Anthony Atala
Yuanyuan Zhang

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite successful approaches to preserve organs, tissues, and isolated cells, the maintenance of stem cell viability and function in body fluids during storage for cell distribution and transportation remains unexplored. The aim of this study was to characterize urine-derived stem cells (USCs) after optimal preservation of urine specimens for up to 24 hours. A total of 415 urine specimens were collected from 12 healthy men (age range 20-54 years old). About 6 × 10(4) cells shed off from the urinary tract system in 24 hours. At least 100 USC clones were obtained from the stored urine specimens after 24 hours and maintained similar biological features to fresh USCs. The stored USCs had a "rice grain" shape in primary culture, and expressed mesenchymal stem cell surface markers, high telomerase activity, and normal karyotypes. Importantly, the preserved cells retained bipotent differentiation capacity. Differentiated USCs expressed myogenic specific proteins and contractile function when exposed to myogenic differentiation medium, and they expressed urothelial cell-specific markers and barrier function when exposed to urothelial differentiation medium. These data demonstrated that up to 75% of fresh USCs can be safely persevered in urine for 24 hours and that these cells stored in urine retain their original stem cell properties, indicating that preserved USCs could be available for potential use in cell-based therapy or clinical diagnosis.

Indexed as

Cell DifferentiationCell ProliferationAdultBlotting, WesternCells, CulturedDesminFlow CytometryHumansKaryotypeKeratinsMaleMesenchymal Stem CellsMicroscopy, Electron, TransmissionMiddle AgedMuscle, SmoothMyosinsDesminKeratinsMyosinsTelomeraseUroplakin Ia

Identifiers

PMID23349776
PMCPMC3548815

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.