ArticlePloS one2013
An improved Ras sensor for highly sensitive and quantitative FRET-FLIM imaging.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Different Ras isoforms regulate synaptic plasticity in opposite directions.The EMBO journal · 2025Article
- Article
- Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines.International journal of molecular sciences · 2020Article
- Rac1 is a downstream effector of PKCα in structural synaptic plasticity.Scientific reports · 2020Article
- Multiple sources of signal amplification within the B-cell Ras/MAPK pathway.Molecular biology of the cell · 2019Article
- Intensiometric biosensors visualize the activity of multiple small GTPases in vivo.Nature communications · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Programmable protein circuits in living cells.Science (New York, N.Y.) · 2018Article
- Noninvasive Imaging of Ras Activity by Monomolecular Biosensor Based on Split-Luciferase Complementary Assay.Scientific reports · 2017Article
- ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.Scientific reports · 2017Article
- RAS Promotes Proliferation and Resistances to Apoptosis in Meningioma.Molecular neurobiology · 2017Article
- Activation of G Proteins by Guanine Nucleotide Exchange Factors Relies on GTPase Activity.PloS one · 2016Article
- A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer.Scientific reports · 2015Article
- Genetically encoded fluorescent biosensors for live-cell visualization of protein phosphorylation.Chemistry & biology · 2014Review
- Neurofibromin is the major ras inactivator in dendritic spines.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Article
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2 authors.
Funding
Abstract
Ras is a signaling protein involved in a variety of cellular processes. Hence, studying Ras signaling with high spatiotemporal resolution is crucial to understanding the roles of Ras in many important cellular functions. Previously, fluorescence lifetime imaging (FLIM) of fluorescent resonance energy transfer (FRET)-based Ras activity sensors, FRas and FRas-F, have been demonstrated to be useful for measuring the spatiotemporal dynamics of Ras signaling in subcellular micro-compartments. However the predominantly nuclear localization of the sensors' acceptor has limited its sensitivity. Here, we have overcome this limitation and developed two variants of the existing FRas sensor with different affinities: FRas2-F (K(d)∼1.7 µM) and FRas2-M (K(d)∼0.5 µM). We demonstrate that, under 2-photon fluorescence lifetime imaging microscopy, FRas2 sensors provide higher sensitivity compared to previous sensors in 293T cells and neurons.
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