ArticleThe Journal of biological chemistry2013
The MukB-ParC interaction affects the intramolecular, not intermolecular, activities of topoisomerase IV.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Mechanism of negative supercoil relaxation by Topoisomerase IV.Nucleic acids research · 2026Article
- Article
- Temporospatial control of topoisomerases by essential cellular processes.Current opinion in microbiology · 2024Review
- Reconstitution of SPO11-dependent double-strand break formation.bioRxiv : the preprint server for biology · 2024Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- The MukB-topoisomerase IV interaction mutually suppresses their catalytic activities.Nucleic acids research · 2022Article
- Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates.Biology · 2021Article
- Article
- Mechanisms for Chromosome Segregation in Bacteria.Frontiers in microbiology · 2021Review
- A topological analysis of difference topology experiments of condensin with topoisomerase II.Biology open · 2020Article
- MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin.eLife · 2018Article
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- The MukB-topoisomerase IV interaction is required for proper chromosome compaction.The Journal of biological chemistry · 2017Article
- The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops.The Journal of biological chemistry · 2017Article
- MukB-mediated Catenation of DNA Is ATP and MukEF Independent.The Journal of biological chemistry · 2016Article
- SMC complexes: from DNA to chromosomes.Nature reviews. Molecular cell biology · 2016Review
- Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.PLoS genetics · 2016Article
- MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.Nature communications · 2016Article
- The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF.Cell reports · 2015Article
- A cell cycle-controlled redox switch regulates the topoisomerase IV activity.Genes & development · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Proper chromosome organization is accomplished through binding of proteins such as condensins that shape the DNA and by modulation of chromosome topology by the action of topoisomerases. We found that the interaction between MukB, the bacterial condensin, and ParC, a subunit of topoisomerase IV, enhanced relaxation of negatively supercoiled DNA and knotting by topoisomerase IV, which are intramolecular DNA rearrangements but not decatenation of multiply linked DNA dimers, which is an intermolecular DNA rearrangement required for proper segregation of daughter chromosomes. MukB DNA binding and a specific chiral arrangement of the DNA was required for topoisomerase IV stimulation because relaxation of positively supercoiled DNA was unaffected. This effect could be attributed to a more effective topological reconfiguration of the negatively supercoiled compared with positively supercoiled DNA by MukB. These data suggest that the MukB-ParC interaction may play a role in chromosome organization rather than in separation of daughter chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.