ArticleNature protocols2013
Incorporation of a viral DNA-packaging motor channel in lipid bilayers for real-time, single-molecule sensing of chemicals and double-stranded DNA.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Review
- Nipple fluid for breast cancer diagnosis using the nanopore of Phi29 DNA-packaging motor.Nanomedicine : nanotechnology, biology, and medicine · 2023Article
- Structure and Dynamics of Thermosensitive pDNA Polyplexes Studied by Time-Resolved Fluorescence Spectroscopy.Biomacromolecules · 2020Article
- Construction of RNA nanotubes.Nano research · 2019Article
- Rapid Nanopore Assay for Carbapenem-ResistantFrontiers in microbiology · 2019Article
- Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.Biomaterials · 2018Article
- Engineering of protein nanopores for sequencing, chemical or protein sensing and disease diagnosis.Current opinion in biotechnology · 2018Review
- Cell-free production of a functional oligomeric form of aThe Journal of biological chemistry · 2017Article
- Construction of an aerolysin nanopore in a lipid bilayer for single-oligonucleotide analysis.Nature protocols · 2017Article
- Building membrane nanopores.Nature nanotechnology · 2017Review
- Channel of viral DNA packaging motor for real time kinetic analysis of peptide oxidation states.Biomaterials · 2017Article
- Three-step channel conformational changes common to DNA packaging motors of bacterial viruses T3, T4, SPP1, and Phi29.Virology · 2017Article
- Oriented single directional insertion of nanochannel of bacteriophage SPP1 DNA packaging motor into lipid bilayer via polar hydrophobicity.Biomaterials · 2016Article
- Fingerprinting of Peptides with a Large Channel of Bacteriophage Phi29 DNA Packaging Motor.Small (Weinheim an der Bergstrasse, Germany) · 2016Article
- Forces from the Portal Govern the Late-Stage DNA Transport in a Viral DNA Packaging Nanomotor.Biophysical journal · 2016Article
- Fabrication of Nanochannels.Materials (Basel, Switzerland) · 2015Review
- Single pore translocation of folded, double-stranded, and tetra-stranded DNA through channel of bacteriophage phi29 DNA packaging motor.Biomaterials · 2015Article
- Stochastic transport through carbon nanotubes in lipid bilayers and live cell membranes.Nature · 2014Article
- Nanobiomotors of archaeal DNA repair machineries: current research status and application potential.Cell & bioscience · 2014Review
- Channel size conversion of Phi29 DNA-packaging nanomotor for discrimination of single- and double-stranded nucleic acids.ACS nano · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Over the past decade, nanopores have rapidly emerged as stochastic biosensors. This protocol describes the cloning, expression and purification of the channel of the bacteriophage phi29 DNA-packaging nanomotor and its subsequent incorporation into lipid membranes for single-pore sensing of double-stranded DNA (dsDNA) and chemicals. The membrane-embedded phi29 nanochannel remains functional and structurally intact under a range of conditions. When ions and macromolecules translocate through this nanochannel, reliable fingerprint changes in conductance are observed. Compared with other well-studied biological pores, the phi29 nanochannel has a larger cross-sectional area, which enables the translocation of dsDNA. Furthermore, specific amino acids can be introduced by site-directed mutagenesis within the large cavity of the channel to conjugate receptors that are able to bind specific ligands or analytes for desired applications. The lipid membrane-embedded nanochannel system has immense potential nanotechnological and biomedical applications in bioreactors, environmental sensing, drug monitoring, controlled drug delivery, early disease diagnosis and high-throughput DNA sequencing. The total time required for completing one round of this protocol is around 1 month.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.