ArticleMicrobial cell factories2013
Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 103 citations in OpenAlex.
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- Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production.Journal of fungi (Basel, Switzerland) · 2024Review
- Microbial valorization of underutilized and nonconventional waste streams.Journal of industrial microbiology & biotechnology · 2022Review
- Lactic acid production - producing microorganisms and substrates sources-state of art.Heliyon · 2020Review
- The Physiological Responses ofMicroorganisms · 2020Article
- Enhancement of gibberellic acid production from3 Biotech · 2020Article
- Engineering the transmission efficiency of the noncyclic glyoxylate pathway for fumarate production inBiotechnology for biofuels · 2020Article
- Model-based temperature control for improving lactic acid production from glycerol.RSC advances · 2019Article
- High-yield production of L-serine from glycerol by engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Article
- Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous cultures of E. coli W.Microbial cell factories · 2018Article
- A modular approach for high-flux lactic acid production from methane in an industrial medium using engineered Methylomicrobium buryatense 5GB1.Journal of industrial microbiology & biotechnology · 2018Article
- Metabolic Engineered Biocatalyst: A Solution for PLA Based Problems.International journal of biomaterials · 2018Review
- Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies.Biotechnology for biofuels · 2018Article
- Assessing glycolytic flux alterations resulting from genetic perturbations in E. coli using a biosensor.Metabolic engineering · 2017Article
- Novel homologous lactate transporter improves L-lactic acid production from glycerol in recombinant strains of Pichia pastoris.Microbial cell factories · 2016Article
- Systems Metabolic Engineering of Escherichia coli.EcoSal Plus · 2016Review
- Toward glycerol biorefinery: metabolic engineering for the production of biofuels and chemicals from glycerol.Biotechnology for biofuels · 2016Review
- Metabolic engineering of Mortierella alpina for arachidonic acid production with glycerol as carbon source.Microbial cell factories · 2015Article
- Synthesis of medium-chain length (C6-C10) fuels and chemicals via β-oxidation reversal in Escherichia coli.Journal of industrial microbiology & biotechnology · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDue to its abundance and low-price, glycerol has become an attractive carbon source for the industrial production of value-added fuels and chemicals. This work reports the engineering of E. coli for the efficient conversion of glycerol into L-lactic acid (L-lactate).
resultsEscherichia coli strains have previously been metabolically engineered for the microaerobic production of D-lactic acid from glycerol in defined media by disrupting genes that minimize the synthesis of succinate, acetate, and ethanol, and also overexpressing the respiratory route of glycerol dissimilation (GlpK/GlpD). Here, further rounds of rationale design were performed on these strains for the homofermentative production of L-lactate, not normally produced in E. coli. Specifically, L-lactate production was enabled by: 1), replacing the native D-lactate specific dehydrogenase with Streptococcus bovis L-lactate dehydrogenase (L-LDH), 2) blocking the methylglyoxal bypass pathways to avoid the synthesis of a racemic mixture of D- and L-lactate and prevent the accumulation of toxic intermediate, methylglyoxal, and 3) the native aerobic L-lactate dehydrogenase was blocked to prevent the undesired utilization of L-lactate. The engineered strain produced 50 g/L of L-lactate from 56 g/L of crude glycerol at a yield 93% of the theoretical maximum and with high optical (99.9%) and chemical (97%) purity.
conclusionsThis study demonstrates the efficient conversion of glycerol to L-lactate, a microbial process that had not been reported in the literature prior to our work. The engineered biocatalysts produced L-lactate from crude glycerol in defined minimal salts medium at high chemical and optical purity.
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