ArticleMicrobial cell factories2013
Repressible promoters - a novel tool to generate conditional mutants in Pichia pastoris.
Article in Microbial cell factories, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Engineering serine metabolism to enhance AOX1 promoter self-induction in formate dehydrogenase-deficient Komagataella phaffii.Microbial cell factories · 2026Article
- Design and construction of light-regulated gene transcription and protein translation systems in yeast P. Pastoris.Journal of advanced research · 2025Article
- Use of the Saccharomycopsis schoenii MET17 promoter for regulated heterologous gene expression.Current genetics · 2024Article
- Genetic tools for metabolic engineering ofEngineering microbiology · 2023Review
- Article
- Cofactor Engineering for Efficient Production of α-Farnesene by Rational Modification of NADPH and ATP Regeneration Pathway inInternational journal of molecular sciences · 2023Article
- Nitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass.Frontiers in bioengineering and biotechnology · 2023Article
- Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.Current genetics · 2021Article
- Understanding and Eliminating the Detrimental Effect of Thiamine Deficiency on the Oleaginous Yeast Yarrowia lipolytica.Applied and environmental microbiology · 2020Article
- A Novel Regulated Hybrid Promoter That Permits Autoinduction of Heterologous Protein Expression in Kluyveromyces lactis.Applied and environmental microbiology · 2019Article
- David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody Fragments.Antibodies (Basel, Switzerland) · 2019Review
- Whole-cell (+)-ambrein production in the yeastMetabolic engineering communications · 2018Article
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.Microbial cell factories · 2017Article
- Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.Microbial cell factories · 2015Article
- L-Methionine repressible promoters for tuneable gene expression in Trichoderma reesei.Microbial cell factories · 2015Article
- Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion.Microbial cell (Graz, Austria) · 2014Article
- Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.Applied microbiology and biotechnology · 2014Review
- Microbials for the production of monoclonal antibodies and antibody fragments.Trends in biotechnology · 2014Review
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundRepressible promoters are a useful tool for down-regulating the expression of genes, especially those that affect cell viability, in order to study cell physiology. They are also popular in biotechnological processes, like heterologous protein production.
resultsHere we present five novel repressible Pichia pastoris promoters of different strength: PSER1, PMET3, PTHR1, PPIS1 and PTHI11. eGFP was expressed under the control of each of these promoters and its fluorescence could be successfully decreased in liquid culture by adding different supplements. We also expressed the essential genes with different native promoter strength, ERO1 and PDI1, under the control of two of the novel promoters. In our experiments, a clear down-regulation of both repressible promoters on transcriptional level could be achieved. Compared to the transcript levels of these two genes when expressed under the control of their native promoters, only ERO1 was significantly down-regulated.
conclusionOur results show that all of the novel promoters can be used for repression of genes in liquid culture. We also came to the conclusion that the choice of the repressible promoter is of particular importance. For a successful repression experiment it is crucial that the native promoter of a gene and the repressible promoter in its non-repressed state are of similar strength.
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