ArticleNucleic acids research2013
IS200/IS605 family single-strand transposition: mechanism of IS608 strand transfer.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Article
- Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection.Communications biology · 2024Article
- Programmable RNA-guided endonucleases are widespread in eukaryotes and their viruses.bioRxiv : the preprint server for biology · 2023Article
- Phylogenetic Relationships among TnpB-Containing Mobile Elements in Six Bacterial Species.Genes · 2023Article
- Single-strand DNA processing: phylogenomics and sequence diversity of a superfamily of potential prokaryotic HuH endonucleases.BMC genomics · 2018Article
- Single strand transposition at the host replication fork.Nucleic acids research · 2016Article
- Real-time transposable element activity in individual live cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Single-Cell (Meta-)Genomics of a Dimorphic Candidatus Thiomargarita nelsonii Reveals Genomic Plasticity.Frontiers in microbiology · 2016Article
- ISC, a Novel Group of Bacterial and Archaeal DNA Transposons That Encode Cas9 Homologs.Journal of bacteriology · 2015Article
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Survey of chimeric IStron elements in bacterial genomes: multiple molecular symbioses between group I intron ribozymes and DNA transposons.Nucleic acids research · 2014Article
- Bacterial insertion sequences: their genomic impact and diversity.FEMS microbiology reviews · 2014Review
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Evolution of REP diversity: a comparative study.BMC genomics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Transposase, TnpA, of the IS200/IS605 family member IS608, catalyses single-strand DNA transposition and is dimeric with hybrid catalytic sites composed of an HUH motif from one monomer and a catalytic Y127 present in an α-helix (αD) from the other (trans configuration). αD is attached to the main body by a flexible loop. Although the reactions leading to excision of a transposition intermediate are well characterized, little is known about the dynamic behaviour of the transpososome that drives this process. We provide evidence strongly supporting a strand transfer model involving rotation of both αD helices from the trans to the cis configuration (HUH and Y residues from the same monomer). Studies with TnpA heterodimers suggest that TnpA cleaves DNA in the trans configuration, and that the catalytic tyrosines linked to the 5'-phosphates exchange positions to allow rejoining of the cleaved strands (strand transfer) in the cis configuration. They further imply that, after excision of the transposon junction, TnpA should be reset to a trans configuration before the cleavage required for integration. Analysis also suggests that this mechanism is conserved among members of the IS200/IS605 family.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.