ArticleJournal of clinical microbiology2013
Comparison of a multiplex real-time PCR assay with a multiplex Luminex assay for influenza virus detection.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-Analysis of the Diagnostic Efficacy of the Luminex xTAG Respiratory Viral Panel FAST v2 Assay for Respiratory Viral Infections.Yonsei medical journal · 2022Pooled it
- Short communication: Dynamic chemical labelling allows the measurement of the paracetamol toxicity biomarker microRNA-122 in hospital clinical laboratories.British journal of clinical pharmacology · 2026Article
- Immunoprofiles of COVID-19 uniquely differentiated from other viruses: A machine learning investigation of multiplex immunoassay data.PNAS nexus · 2024Article
- Recent Advances in Plasma-Engineered Polymers for Biomarker-Based Viral Detection and Highly Multiplexed Analysis.Biosensors · 2022Review
- Advances in the differential molecular diagnosis of vesicular disease pathogens in swine.Frontiers in microbiology · 2022Review
- Article
- Clinical manifestations of influenza and performance of rapid influenza diagnostic test: A university hospital setting.Health science reports · 2021Article
- Gene expression analysis in NSAID-induced rat small intestinal disease model with the intervention of berberine by the liquid chip technology.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Simultaneous Detection of Bluetongue Virus Serotypes Using xMAP Technology.Microorganisms · 2020Article
- Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Detection of Influenza and Other Respiratory Viruses in Air Sampled From a University Campus: A Longitudinal Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Article
- Molecular characterization of influenza viruses from women and infants in Sarlahi, Nepal.Diagnostic microbiology and infectious disease · 2019Article
- A highly multiplexed broad pathogen detection assay for infectious disease diagnostics.PLoS neglected tropical diseases · 2018Article
- Development of Multi-analyte Suspension Assay for Simultaneously Efficient Detection of Avian Influenza Virus A Subtypes.Virologica Sinica · 2018Article
- A Multiplex Asymmetric Reverse Transcription-PCR Assay Combined With an Electrochemical DNA Sensor for Simultaneously Detecting and Subtyping Influenza A Viruses.Frontiers in microbiology · 2018Article
- A multiplex liquid-chip assay based on Luminex xMAP technology for simultaneous detection of six common respiratory viruses.Oncotarget · 2017Article
- Public health risks associated with hepatitis E virus (HEV) as a food-borne pathogen.EFSA journal. European Food Safety Authority · 2017Article
- Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Cascade Invasive Reaction and Nanoparticle Hybridization for Subtyping of Influenza A Virus.Scientific reports · 2017Article
- Detection of influenza viruses by coupling multiplex reverse-transcription loop-mediated isothermal amplification with cascade invasive reaction using nanoparticles as a sensor.International journal of nanomedicine · 2017Article
- Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR-Based Luminex Suspension Microarray Assay.Journal of clinical microbiology · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the development of a multiplex reverse transcription-PCR (RT-PCR) with Luminex microarray hybridization for detection of influenza virus subtypes (FLULUM). Performance of FLULUM was evaluated by comparing it to our real-time RT-PCR influenza virus assay on samples collected during two influenza seasons. Both assays targeted the matrix genes of influenza virus A (FluA M) and influenza virus B (FluB M) and the hemagglutinin genes of seasonal H3N2 (H3) and H1N1 (H1) and 2009 pandemic H1N1 (2009 H1). We evaluated FLULUM on both the Luminex LX200 and the Luminex MagPix instruments. Compared to real-time PCR, FLULUM tested on 259 specimens submitted in the 2010-2011 season showed sensitivities of 97.3% for FluA M, 90.5% for 2009 H1, 96.9% for H3, and 88.9% for FluB M. No specimens were positive for seasonal H1. FLULUM tested on 806 specimens submitted in the 2011-2012 season showed a sensitivity of 100% for FluA M, 89.9% for 2009 H1, 96.4% for H3, and 95.6% for FluB M. No cross-reactivity was observed for other respiratory viruses. Analytical sensitivity was assessed by testing dilutions of specimens with high viral loads. The limits of detection of FLULUM were comparable to those of the real-time PCR assay for FluA M, FluB M, and H3. The limits of detection for seasonal H1 and 2009 H1 were 10-fold higher for the FLULUM assay compared to real-time PCR. The FLULUM is an economic assay with high clinical sensitivity and specificity. It is particularly suited to high-volume detection of influenza viruses.
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