ArticleThe open dentistry journal2012
Metagenomic analysis of subgingival microbiota following non-surgical periodontal therapy: a pilot study.
Article in The open dentistry journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Therapeutic Strategies and Genetic Implications for Periodontal Disease Management: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Using next-generation sequencing to detect oral microbiome change following periodontal interventions: A systematic review.Oral diseases · 2021Pooled it
- No differences in microbiome changes between anti-adhesive and antibacterial ingredients in toothpastes during periodontal therapy.Journal of periodontal research · 2019Trial
- Trial
- The Role of the Oral Microbiome in the Development and Treatment of Periodontal Disease: Functional Meta-Omics Evidence.Current microbiology · 2026Review
- 16S rRNA Gene Amplicon Sequencing of Gut Microbiota in Gestational Diabetes Mellitus and Type 2 Diabetes Mellitus.Journal of diabetes research · 2026Article
- Cytokine release and NLRP3 inflammasome activation induced by low-abundance oral bacterial biofilms.Journal of oral microbiology · 2025Article
- Effect of Periodontal Interventions on Characteristics of the Periodontal Microbial Profile: A Systematic Review and Meta-Analysis.Microorganisms · 2022Article
- Microbiota in Periodontitis: Advances in the Omic Era.Advances in experimental medicine and biology · 2022Review
- The subgingival microbiome associated with periodontitis in type 2 diabetes mellitus.The ISME journal · 2020Article
- Electrospun PLA Fibers Containing Metronidazole for Periodontal Disease.Drug design, development and therapy · 2020Article
- Combination of Zinc Hyaluronate and Metronidazole in a Lipid-Based Drug Delivery System for the Treatment of Periodontitis.Pharmaceutics · 2019Article
- Oral microbiota of periodontal health and disease and their changes after nonsurgical periodontal therapy.The ISME journal · 2018Article
- Signature of Microbial Dysbiosis in Periodontitis.Applied and environmental microbiology · 2017Article
- Microbial profiles at baseline and not the use of antibiotics determine the clinical outcome of the treatment of chronic periodontitis.Scientific reports · 2016Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
This study tested the feasibility of a high throughput metagenomic approach to analyze the pre- and posttreatment of subgingival plaque in two subjects with aggressive periodontitis. DNA was extracted from subgingival samples and subjected to PCR amplification of the c2-c4 regions of the 16S rDNA using primers with bar codes to identify individual samples. The PCR products were pooled and sequenced for the v4 region of the 16S rDNA using the 454 FLX standard platform. The results were analyzed for species/phylotypes in the Human Oral Microbiome Database (HOMD) and Ribosomal Database Project (RDP) database. The sequencing of the amplicons resulted in 24,673 reads and identified 208 species/phylotypes. Of those, 129 species/phylotypes were identified in both patients but their proportions varied. While >120 species/phylotypes were identified in all samples, 28-42 species/phylotypes cumulatively represent 90% of all subgingival bacteria in each sample. The remaining species/phylotypes each constituted ≤0.2% of the total subgingival bacteria. In conclusion, the subgingival microbiota are characterized by high species richness dominated by a few species/ phylotypes. The microbiota changed after periodontal therapy. High throughput metagenomic analysis is applicable to assess the complexity and changes of the subgingival microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.