ArticlePLoS genetics2013
E. coli Fis protein insulates the cbpA gene from uncontrolled transcription.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- The bacterial RNA polymerase-associated CarD protein couples promoter activity to DNA supercoiling.Nature communications · 2026Article
- Spatial Chromosome Organization and Adaptation ofMicroorganisms · 2024Article
- Systems engineering of Escherichia coli for high-level glutarate production from glucose.Nature communications · 2024Article
- When push comes to shove - RNA polymerase and DNA-bound protein roadblocks.Biophysical reviews · 2023Review
- An unexpected abundance of bidirectional promoters withinMicrobiology (Reading, England) · 2023Article
- Genome-wide promoter assembly inGenome research · 2022Article
- Distinct heterochromatin-like domains promote transcriptional memory and silence parasitic genetic elements in bacteria.The EMBO journal · 2022Article
- The quorum sensing transcription factor AphA directly regulates natural competence in Vibrio cholerae.PLoS genetics · 2019Article
- cAMP Receptor Protein Controls Vibrio cholerae Gene Expression in Response to Host Colonization.mBio · 2018Article
- Waking the neighbours: disruption of H-NS repression by overlapping transcription.Molecular microbiology · 2018Article
- Fis is a global regulator critical for modulation of virulence factor production and pathogenicity of Dickeya zeae.Scientific reports · 2018Article
- Spurious transcription and its impact on cell function.Transcription · 2018Article
- The multiple antibiotic resistance operon of enteric bacteria controls DNA repair and outer membrane integrity.Nature communications · 2017Article
- Horizontally acquired AT-rich genes in Escherichia coli cause toxicity by sequestering RNA polymerase.Nature microbiology · 2017Article
- Mapping Topoisomerase IV Binding and Activity Sites on the E. coli Genome.PLoS genetics · 2016Article
- Roles of Nucleoid-Associated Proteins in Stress-Induced Mutagenic Break Repair in Starving Escherichia coli.Genetics · 2015Article
- Dynamic Transcriptional Regulation of Fis in Salmonella During the Exponential Phase.Current microbiology · 2015Article
- DNA recognition by Escherichia coli CbpA protein requires a conserved arginine-minor-groove interaction.Nucleic acids research · 2015Article
- Road rules for traffic on DNA-systematic analysis of transcriptional roadblocking in vivo.Nucleic acids research · 2014Article
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Escherichia coli curved DNA binding protein A (CbpA) is a poorly characterised nucleoid associated factor and co-chaperone. It is expressed at high levels as cells enter stationary phase. Using genetics, biochemistry, and genomics, we have examined regulation of, and DNA binding by, CbpA. We show that Fis, the dominant growth-phase nucleoid protein, prevents CbpA expression in growing cells. Regulation by Fis involves an unusual "insulation" mechanism. Thus, Fis protects cbpA from the effects of a distal promoter, located in an adjacent gene. In stationary phase, when Fis levels are low, CbpA binds the E. coli chromosome with a preference for the intrinsically curved Ter macrodomain. Disruption of the cbpA gene prompts dramatic changes in DNA topology. Thus, our work identifies a novel role for Fis and incorporates CbpA into the growing network of factors that mediate bacterial chromosome structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.