ArticleProceedings of the National Academy of Sciences of the United States of America2013
Bile salt-induced intermolecular disulfide bond formation activates Vibrio cholerae virulence.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 139 citations in OpenAlex.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Nitric oxide promotes rapid development of motility to accelerate biofilm dispersal inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Effects of Bile on PathogenicBiomolecules · 2025Article
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- In Vivo Nitrosative Stress-Induced Expression of a Photolyase Promotes Vibrio cholerae Environmental Blue Light Resistance.Molecular microbiology · 2025Article
- Pathogenicity and virulence ofVirulence · 2024Review
- AcrAB Efflux Pump Plays a Crucial Role in Bile Salts Resistance and Pathogenesis ofAntibiotics (Basel, Switzerland) · 2024Article
- Article
- The two-component system TtrRS boosts Vibrio parahaemolyticus colonization by exploiting sulfur compounds in host gut.PLoS pathogens · 2024Article
- The intersection between host-pathogen interactions and metabolism during Vibrio cholerae infection.Current opinion in microbiology · 2024Review
- Identification of critical amino acids in the DNA binding domain of LuxO: Lessons from a constitutive active LuxO.PloS one · 2024Article
- Gut microbiota of Pacific white shrimp (Microbiology spectrum · 2023Article
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- Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistance.PLoS genetics · 2023Article
- Host-derived O-glycans inhibit toxigenic conversion by a virulence-encoding phage in Vibrio cholerae.The EMBO journal · 2023Article
- Structural Insights into Regulation of Vibrio Virulence Gene Networks.Advances in experimental medicine and biology · 2023Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Vibrio cholerae RND efflux systems: mediators of stress responses, colonization and pathogenesis.Frontiers in cellular and infection microbiology · 2023Review
- Review
- Intestinal colonization againstAIMS microbiology · 2023Review
44 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
To be successful pathogens, bacteria must often restrict the expression of virulence genes to host environments. This requires a physical or chemical marker of the host environment as well as a cognate bacterial system for sensing the presence of a host to appropriately time the activation of virulence. However, there have been remarkably few such signal-sensor pairs identified, and the molecular mechanisms for host-sensing are virtually unknown. By directly applying a reporter strain of Vibrio cholerae, the causative agent of cholera, to a thin layer chromatography (TLC) plate containing mouse intestinal extracts, we found two host signals that activate virulence gene transcription. One of these was revealed to be the bile salt taurocholate. We then show that a set of bile salts cause dimerization of the transmembrane transcription factor TcpP by inducing intermolecular disulfide bonds between cysteine (C)-207 residues in its periplasmic domain. Various genetic and biochemical analyses led us to propose a model in which the other cysteine in the periplasmic domain, C218, forms an inhibitory intramolecular disulfide bond with C207 that must be isomerized to form the active C207-C207 intermolecular bond. We then found bile salt-dependent effects of these cysteine mutations on survival in vivo, correlating to our in vitro model. Our results are a demonstration of a mechanism for direct activation of the V. cholerae virulence cascade by a host signal molecule. They further provide a paradigm for recognition of the host environment in pathogenic bacteria through periplasmic cysteine oxidation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.