ArticleInvestigative ophthalmology & visual science2013
Disruption and destabilization of meibomian lipid films caused by increasing amounts of ceramides and cholesterol.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 53 citations in OpenAlex.
- Sphingolipids in human disease: organ-specific pathologies, chain-length-dependent effects, and translational implications.Journal of translational medicine · 2026Review
- A Novel Rat Model for Human Meibomian Gland Dysfunction and Ocular Rosacea.Investigative ophthalmology & visual science · 2026Article
- Clinical and Meibomian Lipidomic Changes After Combined Intense Pulsed Light and Triamcinolone Acetonide Therapy for Chalazion: A Retrospective Single-Arm Pre-Post Case Series.Journal of ophthalmology · 2026Article
- A Randomised Pilot Trial to Demonstrate the Feasibility of a Prototype Electronic Heating Device in Patients with Meibomian Gland Dysfunction.Biomedicines · 2025Article
- Surface Rheological Properties and Microstructures of DPPC/POPC Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- How Eyelid Changes May Impact on Tears.Journal of clinical medicine · 2024Review
- Physiological Effects of Soat1 Inactivation on Homeostasis of the Mouse Ocular Surface.Investigative ophthalmology & visual science · 2024Article
- Unraveling Complex Hysteresis Phenomenon in 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine Monolayer: Insight into Factors Influencing Surface Dynamics.International journal of molecular sciences · 2023Article
- Comparative Biophysical Study of Meibomian Lipids of Wild Type and Soat1-Null Mice: Implications to Meibomian Gland Dysfunction and Dry Eye Disease.Investigative ophthalmology & visual science · 2023Article
- Impact of Exposomes on Ocular Surface Diseases.International journal of molecular sciences · 2023Review
- The role of sphingolipids in meibomian gland dysfunction and ocular surface inflammation.The ocular surface · 2022Review
- Association of Meibomian Gland Dysfunction with Oral Statin Use.Journal of clinical medicine · 2022Article
- Relationships between ocular surface sphingomyelinases, Meibum and Tear Sphingolipids, and clinical parameters of meibomian gland dysfunction.The ocular surface · 2022Article
- Biophysical properties of tear film lipid layer II. Polymorphism of FAHFA.Biophysical journal · 2022Article
- Statin Use Is Associated With a Lower Risk of Blepharitis: A Population-Based Study.Frontiers in medicine · 2022Article
- Meibum sphingolipid composition is altered in individuals with meibomian gland dysfunction-a side by side comparison of Meibum and Tear Sphingolipids.The ocular surface · 2022Article
- Novel Lipids of the Rabbit Harderian Gland Improve Tear Stability in an Animal Model of Dry Eye Disease.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2021Article
- Effects of Aging on Human Meibum.Investigative ophthalmology & visual science · 2021Article
- Differential effects of dietary cholesterol and triglycerides on the lipid homeostasis in Meibomian glands.The Journal of steroid biochemistry and molecular biology · 2021Article
- Tear Lipocalin and Lipocalin-Interacting Membrane Receptor.Frontiers in physiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeWe evaluated quantitatively direct effects of ceramide (Cer) and free cholesterol (FC) on meibomian lipid films (MLF) using a Langmuir trough (LT) and a Brewster angle microscope (BAM).
methodsMeibum was obtained from healthy volunteers. A series of mixtures of meibum with Cer or FC (mixed MLF) taken in different ratios were tested. Standard rheologic parameters, such as elasticity and hysteresis of MLF, were computed. BAM was used to study the morphology of MLF.
resultsPure MLF were capable of withstanding multiple compression/expansion cycles with little hysteresis observed (1.9 J/G meibum). The films made of either pure CER or pure FC were clearly collapsible, and had much higher rigidity and hysteresis than pure meibum. Adding progressively higher amounts of CER or FC to meibum had a strong impact on the rigidity, stability, and morphology of the mixed MLF: their hysteresis increased many fold compared to pure meibum. A concomitant increase in the rigidity and collapsibility of the mixed MLF was observed.
conclusionsCer and FC changed the surface properties of mixed MLF in a way that implied their destabilization and/or disruption. One of the mechanisms that might lead to these effects is strong aggregation of meibum lipids with FC or Cer that leads to the formation of smaller particles of meibum surrounded by a thinner layer of FC or Cer. As Cer and FC can be elevated in meibum and the tear film because of certain pathologic processes, or can be of exogenous nature, our results can explain (partially) a less stable tear film in those subjects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.