ArticleMolecular bioSystems2013
Fluorogenic label to quantify the cytosolic delivery of macromolecules.
Article in Molecular bioSystems, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Protein Modifications for Cellular Protein Delivery.Chemical reviews · 2026Review
- Evaluation of the Cytosolic Uptake of HaloTag Using a pH-Sensitive Dye.ACS chemical biology · 2024Article
- Selective Cleavage at CCA Ends and Anticodon Loops of tRNAs by Stress-Induced RNases.Frontiers in molecular biosciences · 2022Article
- Understanding Cell Penetration of Cyclic Peptides.Chemical reviews · 2019Review
- Esterification Delivers a Functional Enzyme into a Human Cell.ACS chemical biology · 2019Article
- Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization.Bioconjugate chemistry · 2019Review
- Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins.ACS central science · 2018Article
- Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor.RNA (New York, N.Y.) · 2018Article
- Enzyme-Activated Fluorogenic Probes for Live-Cell and in Vivo Imaging.ACS chemical biology · 2018Review
- Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus.Nucleus (Austin, Tex.) · 2018Article
- Knockout of the Ribonuclease Inhibitor Gene Leaves Human Cells Vulnerable to Secretory Ribonucleases.Biochemistry · 2016Article
- Fluorescence correlation spectroscopy reveals highly efficient cytosolic delivery of certain penta-arg proteins and stapled peptides.Journal of the American Chemical Society · 2015Article
- Contribution of electrostatics to the binding of pancreatic-type ribonucleases to membranes.Biochemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coliβ-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal β-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.