ArticleMolecular systems biology2013
Autonomous bacterial localization and gene expression based on nearby cell receptor density.
Article in Molecular systems biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Engineering Ag43 Signal Peptides with Bacterial Display and Selection.Methods and protocols · 2022Article
- The current status of nanotechnological approaches to therapy and drug delivery in otolaryngology: A contemporary review.Laryngoscope investigative otolaryngology · 2022Review
- Engineered bacteria as drug delivery vehicles: Principles and prospects.Engineering microbiology · 2022Review
- The Primary Physiological Roles of Autoinducer 2 inMicroorganisms · 2021Article
- Homologous Quorum Sensing Regulatory Circuit: A Dual-Input Genetic Controller for Modulating Quorum Sensing-Mediated Protein Expression inACS synthetic biology · 2020Article
- Building sophisticated sensors of extracellular cues that enable mammalian cells to work as "doctors" in the body.Cellular and molecular life sciences : CMLS · 2020Review
- Programmable Synthetic Protein Circuits for the Identification and Suppression of Hepatocellular Carcinoma.Molecular therapy oncolytics · 2020Article
- A redox-based electrogenetic CRISPR system to connect with and control biological information networks.Nature communications · 2020Article
- Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition.Nature communications · 2019Article
- Bacteria Floc, but Do They Flock? Insights from Population Interaction Models of Quorum Sensing.mBio · 2019Article
- Plasmid-encoded protein attenuates Escherichia coli swimming velocity and cell growth, not reprogrammed regulatory functions.Biotechnology progress · 2019Article
- Bottom-Up Approaches to Synthetic Cooperation in Microbial Communities.Life (Basel, Switzerland) · 2019Review
- A platform of genetically engineered bacteria as vehicles for localized delivery of therapeutics: Toward applications for Crohn's disease.Bioengineering & translational medicine · 2018Article
- Development of Cell-Based Sentinels for Nitric Oxide: Ensuring Marker Expression and Unimodality.ACS synthetic biology · 2018Article
- Focusing quorum sensing signalling by nano-magnetic assembly.Environmental microbiology · 2018Article
- Biofabricating Functional Soft Matter Using Protein Engineering to Enable Enzymatic Assembly.Bioconjugate chemistry · 2018Review
- Designer cells programming quorum-sensing interference with microbes.Nature communications · 2018Article
- The potential roles of bacteria to improve radiation treatment outcome.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2018Review
- Engineering bacterial motility towards hydrogen-peroxide.PloS one · 2018Article
- A new design for an artificial cell: polymer microcapsules with addressable inner compartments that can harbor biomolecules, colloids or microbial species.Chemical science · 2017Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli were genetically modified to enable programmed motility, sensing, and actuation based on the density of features on nearby surfaces. Then, based on calculated feature density, these cells expressed marker proteins to indicate phenotypic response. Specifically, site-specific synthesis of bacterial quorum sensing autoinducer-2 (AI-2) is used to initiate and recruit motile cells. In our model system, we rewired E. coli's AI-2 signaling pathway to direct bacteria to a squamous cancer cell line of head and neck (SCCHN), where they initiate synthesis of a reporter (drug surrogate) based on a threshold density of epidermal growth factor receptor (EGFR). This represents a new type of controller for targeted drug delivery as actuation (synthesis and delivery) depends on a receptor density marking the diseased cell. The ability to survey local surfaces and initiate gene expression based on feature density represents a new area-based switch in synthetic biology that will find use beyond the proposed cancer model here.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.