ArticleThe EMBO journal2013
Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 97 citations in OpenAlex.
- Sororin locks the DNA-exit gate of cohesin to preserve sister-chromatid cohesion.Nature communications · 2026Article
- A chromatin-remodeling-independent role for ATRX in protecting centromeric cohesion.The EMBO journal · 2025Article
- Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin.The EMBO journal · 2024Article
- Molecular mechanism and functional significance of Wapl interaction with the Cohesin complex.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cohesin contributes to transcriptional repression of stage-specific genes in the human malaria parasite.EMBO reports · 2023Article
- A dual-function SNF2 protein drives chromatid resolution and nascent transcripts removal in mitosis.EMBO reports · 2023Article
- Genome control by SMC complexes.Nature reviews. Molecular cell biology · 2023Review
- DNA segment capture by Smc5/6 holocomplexes.Nature structural & molecular biology · 2023Article
- Article
- Genome folding through loop extrusion by SMC complexes.Nature reviews. Molecular cell biology · 2021Review
- Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space.Essays in biochemistry · 2020Review
- Biology and Physics of Heterochromatin-Cells · 2020Review
- A role of the Nse4 kleisin and Nse1/Nse3 KITE subunits in the ATPase cycle of SMC5/6.Scientific reports · 2020Article
- The structure of the cohesin ATPase elucidates the mechanism of SMC-kleisin ring opening.Nature structural & molecular biology · 2020Article
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- Article
- Calcineurin-dependent Protein Phosphorylation Changes During Egg Activation inMolecular & cellular proteomics : MCP · 2019Article
- Phosphatases in Mitosis: Roles and Regulation.Biomolecules · 2019Review
- Induced aneuploidy in neural stem cells triggers a delayed stress response and impairs adult life span in flies.PLoS biology · 2019Article
- Interphase cohesin regulation ensures mitotic fidelity after genome reduplication.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.