ArticleNature communications2013
Evolution of the protein stoichiometry in the L12 stalk of bacterial and organellar ribosomes.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Stoichiometry-induced differential selection on codon optimization among ribosomal protein genes in bacterial species.Molecular biology and evolution · 2026Article
- Ribosomal L12 stalks recruit elongation factors to speed protein synthesis in Escherichia coli.Communications biology · 2025Article
- Phosphorylation of P-stalk proteins defines the ribosomal state for interaction with auxiliary protein factors.EMBO reports · 2024Article
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Single-particle mass analysis of intact ribosomes by mass photometry and Orbitrap-based charge detection mass spectrometry.iScience · 2021Article
- Structural basis for late maturation steps of the human mitoribosomal large subunit.Nature communications · 2021Article
- Article
- Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins.PloS one · 2020Article
- Probing the dynamic stalk region of the ribosome using solution NMR.Scientific reports · 2019Article
- How Ricin Damages the Ribosome.Toxins · 2019Review
- Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening.Molecular & cellular proteomics : MCP · 2018Article
- Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods.Nature communications · 2018Article
- Multiplication of Ribosomal P-Stalk Proteins Contributes to the Fidelity of Translation.Molecular and cellular biology · 2017Article
- Genome-wide mRNA processing in methanogenic archaea reveals post-transcriptional regulation of ribosomal protein synthesis.Nucleic acids research · 2017Article
- From molecular chaperones to membrane motors: through the lens of a mass spectrometrist.Biochemical Society transactions · 2017Article
- Review: Translational GTPases.Biopolymers · 2016Review
- Activities of the peptidyl transferase center of ribosomes lacking protein L27.RNA (New York, N.Y.) · 2015Article
- Translation initiation factor 3 families: what are their roles in regulating cyanobacterial and chloroplast gene expression?Photosynthesis research · 2015Review
- Studying macromolecular complex stoichiometries by peptide-based mass spectrometry.Proteomics · 2015Review
- Functional Importance of Mobile Ribosomal Proteins.BioMed research international · 2015Review
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of ribosomes and translation factors is central for understanding the origin of life. Recruitment of translation factors to bacterial ribosomes is mediated by the L12 stalk composed of protein L10 and several copies of protein L12, the only multi-copy protein of the ribosome. Here we predict stoichiometries of L12 stalk for >1,200 bacteria, mitochondria and chloroplasts by a computational analysis, and validate the predictions by quantitative mass spectrometry. The majority of bacteria have L12 stalks allowing for binding of four or six copies of L12, largely independent of the taxonomic group or living conditions of the bacteria, whereas some cyanobacteria have eight copies. Mitochondrial and chloroplast ribosomes can accommodate six copies of L12. The last universal common ancestor probably had six molecules of L12 molecules bound to L10. Changes of the stalk composition provide a unique possibility to trace the evolution of protein components of the ribosome.
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