ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2013
A comparison between manual and automated evaluations of tissue microarray patterns of protein expression.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
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- Machine Learning for Digital Scoring of PRMT6 in Immunohistochemical Labeled Lung Cancer.Cancers · 2023Article
- Aberrant expression of RSK1 characterizes high-grade gliomas with immune infiltration.Molecular oncology · 2020Article
- Computer-assisted image analysis of the tumor microenvironment on an oral tongue squamous cell carcinoma tissue microarray.Clinical and translational radiation oncology · 2019Article
- Polysome Profiling of a Human Glioblastoma Reveals Intratumoral Heterogeneity.International journal of molecular sciences · 2019Article
- Overexpression of mTOR and p(240-244)S6 in IDH1 Wild-Type Human Glioblastomas Is Predictive of Low Survival.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
- Evaluation of Akt and RICTOR Expression Levels in Astrocytomas of All Grades.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- A novel immunofluorescent assay to investigate oxidative phosphorylation deficiency in mitochondrial myopathy: understanding mechanisms and improving diagnosis.Scientific reports · 2015Article
- Disruption of prion protein-HOP engagement impairs glioblastoma growth and cognitive decline and improves overall survival.Oncogene · 2015Article
- Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review.Diagnostic pathology · 2014Review
- Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis.Oncology letters · 2014Article
- Somatostatin receptor immunohistochemistry in neuroendocrine tumors: comparison between manual and automated evaluation.International journal of clinical and experimental pathology · 2014Article
- Epithelial-mesenchymal transition-like events in vulvar cancer and its relation with HPV.British journal of cancer · 2013Article
- Detection of Putative Stem-cell Markers in Invasive Ductal Carcinoma of the Breast by Immunohistochemistry: Does It Improve Prognostic/Predictive Assessments?Applied immunohistochemistry & molecular morphology : AIMMArticle
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8 authors.
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Abstract
Tissue microarray technology enables us to evaluate the pattern of protein expression in large numbers of samples. However, manual data acquisition and analysis still represent a challenge because they are subjective and time-consuming. Automated analysis may thus increase the speed and reproducibility of evaluation. However, the reliability of automated analysis systems should be independently evaluated. Herein, the expression of phosphorylated AKT and mTOR was determined by ScanScope XT (Aperio; Vista, CA) and ACIS III (Dako; Glostrup, Denmark) and compared with the manual analysis by two observers. The percentage of labeled pixels or nuclei analysis had a good correlation between human observers and automated systems (κ = 0.855 and 0.879 for ScanScope vs. observers and κ = 0.765 and 0.793 for ACIS III vs. observers). The intensity of labeling determined by ScanScope was also correlated with that found by the human observers (correlation index of 0.946 and 0.851 for pAKT and 0.851 and 0.875 for pmTOR). However, the correlation between ACIS III and human observation varied for labeling intensity and was considered poor in some cases (correlation index of 0.718 and 0.680 for pAKT and 0.223 and 0.225 for pmTOR). Thus, the percentage of positive pixels or nuclei determination was satisfactorily performed by both systems; however, labeling intensity was better identified by ScanScope XT.
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