ReviewProteomics. Clinical applications2013
Complete solubilization of formalin-fixed, paraffin-embedded tissue may improve proteomic studies.
Review in Proteomics. Clinical applications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A Fast-Tracking Sample Preparation Protocol for Proteomics of Formalin-Fixed Paraffin-Embedded Tumor Tissues.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens.Clinical proteomics · 2022Article
- Next-generation sequencing: A new avenue to understand viral RNA-protein interactions.The Journal of biological chemistry · 2022Review
- An efficient procedure for the recovery of DNA from formalin-fixed paraffin-embedded tissue sections.Biology methods & protocols · 2022Article
- Target Score-A Proteomics Data Selection Tool Applied to Esophageal Cancer Identifies GLUT1-Sialyl Tn Glycoforms as Biomarkers of Cancer Aggressiveness.International journal of molecular sciences · 2021Article
- Protein Extraction From FFPE Kidney Tissue Samples: A Review of the Literature and Characterization of Techniques.Frontiers in medicine · 2021Article
- Novel Proteome Extraction Method Illustrates a Conserved Immunological Signature of MSI-H Colorectal Tumors.Molecular & cellular proteomics : MCP · 2020Article
- Quantitative Profiling of the Human Substantia Nigra Proteome from Laser-capture Microdissected FFPE Tissue.Molecular & cellular proteomics : MCP · 2020Article
- Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C δ Pathway in a Subset of Triple-Negative Breast Cancers.The American journal of pathology · 2020Article
- High-throughput proteomic analysis of FFPE tissue samples facilitates tumor stratification.Molecular oncology · 2019Article
- Residual tissue repositories as a resource for population-based cancer proteomic studies.Clinical proteomics · 2018Article
- Second-generation method for analysis of chromatin binding with formaldehyde-cross-linking kinetics.The Journal of biological chemistry · 2017Article
- Tumour heterogeneity: principles and practical consequences.Virchows Archiv : an international journal of pathology · 2016Review
- Formaldehyde crosslinking: a tool for the study of chromatin complexes.The Journal of biological chemistry · 2015Review
- Quantitative proteomic analysis of formalin-fixed, paraffin-embedded clear cell renal cell carcinoma tissue using stable isotopic dimethylation of primary amines.BMC genomics · 2015Article
- Reverse Phase Protein Arrays-Quantitative Assessment of Multiple Biomarkers in Biopsies for Clinical Use.Microarrays (Basel, Switzerland) · 2015Review
- Improving the Proteomic Analysis of Archival Tissue by Using Pressure-Assisted Protein Extraction: A Mechanistic Approach.Journal of proteomics & bioinformatics · 2014Article
- Applications of mass spectrometry for quantitative protein analysis in formalin-fixed paraffin-embedded tissues.Proteomics · 2014Review
- Efficient extraction of proteins from formalin-fixed paraffin-embedded tissues requires higher concentration of tris(hydroxymethyl)aminomethane.Clinical proteomics · 2014Article
- Practicing pathology in the era of big data and personalized medicine.Applied immunohistochemistry & molecular morphology : AIMM · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Tissue-based proteomic approaches (tissue proteomics) are essential for discovering and evaluating biomarkers for personalized medicine. In any proteomics study, the most critical issue is sample extraction and preparation. This problem is especially difficult when recovering proteins from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, improving and standardizing protein extraction from FFPE tissue is a critical need because of the millions of archival FFPE tissues available in tissue banks worldwide. Recent progress in the application of heat-induced antigen retrieval principles for protein extraction from FFPE tissue has resulted in a number of published FFPE tissue proteomics studies. However, there is currently no consensus on the optimal protocol for protein extraction from FFPE tissue or accepted standards for quantitative evaluation of the extracts. Standardization is critical to ensure the accurate evaluation of FFPE protein extracts by proteomic methods such as reverse phase protein arrays, which is now in clinical use. In our view, complete solubilization of FFPE tissue samples is the best way to achieve the goal of standardizing the recovery of proteins from FFPE tissues. However, further studies are recommended to develop standardized protein extraction methods to ensure quantitative and qualitative reproducibility in the recovery of proteins from FFPE tissues.
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