ArticleALTEX2013
Perspectives on validation of high-throughput assays supporting 21st century toxicity testing.
Article in ALTEX, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A genomics-based framework for identifying biomarkers of human neurodevelopmental toxicity.Reproductive toxicology (Elmsford, N.Y.) · 2016Pooled it
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- Adverse Outcome Pathways as Versatile Tools in Liver Toxicity Testing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity.Current research in toxicology · 2022Article
- Scientific Validation of Human Neurosphere Assays for Developmental Neurotoxicity Evaluation.Frontiers in toxicology · 2022Article
- In vitro screening for chemical inhibition of the iodide recycling enzyme, iodotyrosine deiodinase.Toxicology in vitro : an international journal published in association with BIBRA · 2021Article
- Improvements in Smartphone and Night Vision Imaging Technologies Enable Low Cost, On-Site Assays of Bioluminescent Cells.Frontiers in bioengineering and biotechnology · 2021Article
- Methods, models, mechanisms and metadata: Introducing the Nanotoxicology collection at F1000Research.F1000Research · 2021Article
- The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods.Archives of toxicology · 2020Article
- Data Mining Approach for Extraction of Useful Information About Biologically Active Compounds from Publications.Journal of chemical information and modeling · 2019Article
- Screening the ToxCast Phase 1, Phase 2, and e1k Chemical Libraries for Inhibitors of Iodothyronine Deiodinases.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Multi-Behavioral Endpoint Testing of an 87-Chemical Compound Library in Freshwater Planarians.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Perspectives onApplied in vitro toxicology · 2018Review
- Investigating the establishment of primary cultures of hemocytes from Mytilus edulis.Cytotechnology · 2018Article
- The use of purified rat Leydig cells complements the H295R screen to detect chemical-induced alterations in testosterone production.Biology of reproduction · 2018Article
- The US Federal Tox21 Program: A strategic and operational plan for continued leadership.ALTEX · 2018Article
- LUCS (Light-Up Cell System), a universal high throughput assay for homeostasis evaluation in live cells.Scientific reports · 2017Article
- On selecting a minimal set of in vitro assays to reliably determine estrogen agonist activity.Regulatory toxicology and pharmacology : RTP · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors.
Funding
Abstract
In vitro high-throughput screening (HTS) assays are seeing increasing use in toxicity testing. HTS assays can simultaneously test many chemicals but have seen limited use in the regulatory arena, in part because of the need to undergo rigorous, time-consuming formal validation. Here we discuss streamlining the validation process, specifically for prioritization applications. By prioritization, we mean a process in which less complex, less expensive, and faster assays are used to prioritize which chemicals are subjected first to more complex, expensive, and slower guideline assays. Data from the HTS prioritization assays is intended to provide a priori evidence that certain chemicals have the potential to lead to the types of adverse effects that the guideline tests are assessing. The need for such prioritization approaches is driven by the fact that there are tens of thousands of chemicals to which people are exposed, but the yearly throughput of most guideline assays is small in comparison. The streamlined validation process would continue to ensure the reliability and relevance of assays for this application. We discuss the following practical guidelines: (1) follow current validation practice to the extent possible and practical; (2) make increased use of reference compounds to better demonstrate assay reliability and relevance; (3) de-emphasize the need for cross-laboratory testing; and (4) implement a web-based, transparent, and expedited peer review process.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.