ArticleBiology open2013
In vivo genetic manipulation of cortical progenitors in gyrencephalic carnivores using in utero electroporation.
Article in Biology open, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 62 citations in OpenAlex.
- TH/TRs-COL11A2 Axis Mediates Loss of a Differentiated Astrocyte State in Hypogyrified Brains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Investigation of the mechanisms underlying the development and evolution of the mammalian cerebrum using gyrencephalic ferrets.Frontiers in cell and developmental biology · 2026Review
- Gene regulatory landscape of cerebral cortex folding.Science advances · 2024Article
- Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification.Journal of visualized experiments : JoVE · 2023Article
- Embryonic mouse medial neocortex as a model system for studying the radial glial scaffold in fetal human neocortex.Journal of neural transmission (Vienna, Austria : 1996) · 2023Article
- Genetically encoded fluorescent sensors for imaging neuronal dynamics in vivo.Journal of neurochemistry · 2023Review
- Orchestrated neuronal migration and cortical folding: A computational and experimental study.PLoS computational biology · 2022Article
- Investigation of the Mechanisms Underlying the Development and Evolution of the Cerebral Cortex Using Gyrencephalic Ferrets.Frontiers in cell and developmental biology · 2022Review
- Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- The expression of aristaless-related homeobox in neural progenitors of gyrencephalic carnivore ferrets.Biochemistry and biophysics reports · 2021Article
- Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation.Scientific reports · 2021Article
- Roots of the Malformations of Cortical Development in the Cell Biology of Neural Progenitor Cells.Frontiers in neuroscience · 2021Review
- The Ferret as a Model System for Neocortex Development and Evolution.Frontiers in cell and developmental biology · 2021Review
- A discrete subtype of neural progenitor crucial for cortical folding in the gyrencephalic mammalian brain.eLife · 2020Article
- The origin and development of subcortical U-fibers in gyrencephalic ferrets.Molecular brain · 2020Article
- Follow-up study of subventricular zone progenitors with multiple rounds of cell division during sulcogyrogenesis in the ferret cerebral cortex.IBRO reports · 2019Article
- Genes and Mechanisms Involved in the Generation and Amplification of Basal Radial Glial Cells.Frontiers in cellular neuroscience · 2019Review
- Genetic Modification of Brain Organoids.Frontiers in cellular neuroscience · 2019Article
- Malformations of Human Neocortex in Development - Their Progenitor Cell Basis and Experimental Model Systems.Frontiers in cellular neuroscience · 2019Review
- Human-specificeLife · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brain structures such as the outer subventricular zone (OSVZ) and the inner fiber layer (IFL) in the developing cerebral cortex are especially prominent in higher mammals. However, the molecular mechanisms underlying the formation of the OSVZ are still largely unknown, mainly because genetic manipulations that can be applied to the OSVZ in higher mammals had been poorly available. Here we developed and validated a rapid and efficient genetic manipulation technique for germinal zones including the OSVZ using in utero electroporation in developing gyrencephalic carnivore ferrets. We also determined the optimal conditions for using in utero electroporation to express transgenes in germinal zones. Using our electroporation procedure, the morphology of GFP-positive cells in the OSVZ was clearly visible even without immunostaining, and multiple genes were efficiently co-expressed in the same cells. Furthermore, we uncovered that fibers, which seemed to correspond to those in the IFL of monkeys, also existed in ferrets, and were derived from newly generated cortical neurons. Our technique promises to be a powerful tool for investigating the fundamental mechanisms underlying the formation and abnormalities of the cerebral cortex in higher mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.