ArticlePloS one2013
Validation of a commercially available indirect ELISA using a nucleocapside recombinant protein for detection of Schmallenberg virus antibodies.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Serological prevalence of the Schmallenberg virus in domestic and wild hosts worldwide: a systematic review and meta-analysis.Frontiers in veterinary science · 2024Pooled it
- Seroprevalence and risk factors associated with bluetongue and Schmallenberg virus infections in domestic small ruminants in Türkiye.Tropical animal health and production · 2026Article
- Spatial Modelling of Environmental Risk Factors Influencing Schmallenberg Virus Exposure in German Sheep.Transboundary and emerging diseases · 2026Article
- Mysterious Oropouche virus: Transmission, symptoms, and control.Infectious medicine · 2025Review
- Schmallenberg virus epidemiology and regional control strategies: diagnostics, vaccines, and vector management.Frontiers in cellular and infection microbiology · 2025Review
- The Adaptive Immune Response againstViruses · 2024Review
- Article
- Generation of Stable Cell Lines Expressing Akabane Virus N Protein and Insight into Its Function in Viral Replication.Pathogens (Basel, Switzerland) · 2023Article
- Monoclonal antibodies to Cache Valley virus for serological diagnosis.PLoS neglected tropical diseases · 2022Article
- Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods.Frontiers in molecular biosciences · 2021Review
- Review
- Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum.BMC veterinary research · 2019Article
- Immunogenicity and efficacy of Schmallenberg virus envelope glycoprotein subunit vaccines.Journal of veterinary science · 2019Article
- Orthobunyavirus spike architecture and recognition by neutralizing antibodies.Nature communications · 2019Article
- International proficiency trial demonstrates reliable Schmallenberg virus infection diagnosis in endemic and non-affected countries.PloS one · 2019Article
- Schmallenberg virus: a systematic international literature review (2011-2019) from an Irish perspective.Irish veterinary journal · 2019Review
- Neutralizing antibodies against Simbu serogroup viruses in cattle and sheep, Nigeria, 2012-2014.BMC veterinary research · 2018Article
- Preliminary serological evidence for Schmallenberg virus infection in China.Tropical animal health and production · 2018Article
- Test selection for antibody detection according to the seroprevalence level of Schmallenberg virus in sheep.PloS one · 2018Article
- Generation and characterization of a potentially applicable Vero cell line constitutively expressing the Schmallenberg virus nucleocapsid protein.Cytotechnology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A newly developed Enzym Like Immuno Sorbant Assay (ELISA) based on the recombinant nucleocapsid protein (N) of Schmallenberg virus (SBV) was evaluated and validated for the detection of SBV-specific IgG antibodies in ruminant sera by three European Reference Laboratories. Validation data sets derived from sheep, goat and bovine sera collected in France and Germany (n = 1515) in 2011 and 2012 were categorized according to the results of a virus neutralization test (VNT) or an indirect immuno-fluorescence assay (IFA). The specificity was evaluated with 1364 sera from sheep, goat and bovine collected in France and Belgium before 2009. Overall agreement between VNT and ELISA was 98.9% and 98.3% between VNT and IFA, indicating a very good concordance between the different techniques. Although cross-reactions with other Orthobunyavirus from the Simbu serogroup viruses might occur, it is a highly sensitive, specific and robust ELISA-test validated to detect anti-SBV antibodies. This test can be applied for SBV sero-diagnostics and disease-surveillance studies in ruminant species in Europe.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.