ArticlePloS one2013
Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Trypanosome bloodstream-specific flagellum attachment proteins can mediate attachment in an insect surface coat environment.Journal of cell science · 2026Article
- Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei.Nature microbiology · 2023Article
- Subcellular protein localisation ofWellcome open research · 2023Article
- Visualisation of experimentally determined and predicted protein N-glycosylation and predicted glycosylphosphatidylinositol anchor addition inWellcome open research · 2022Article
- Nucleotide sugar biosynthesis occurs in the glycosomes of procyclic and bloodstream form Trypanosoma brucei.PLoS neglected tropical diseases · 2021Article
- Touching the Surface: Diverse Roles for the Flagellar Membrane in Kinetoplastid Parasites.Microbiology and molecular biology reviews : MMBR · 2020Review
- The Protein Neddylation Pathway in Trypanosoma brucei: FUNCTIONAL CHARACTERIZATION AND SUBSTRATE IDENTIFICATION.The Journal of biological chemistry · 2017Article
- The Flagellum Attachment Zone: 'The Cellular Ruler' of Trypanosome Morphology.Trends in parasitology · 2016Review
- Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone.Journal of cell science · 2016Article
- Assembly and maintenance of the flagellum attachment zone filament in Trypanosoma brucei.Journal of cell science · 2015Article
- Architecture of a Host-Parasite Interface: Complex Targeting Mechanisms Revealed Through Proteomics.Molecular & cellular proteomics : MCP · 2015Article
- Motility and more: the flagellum of Trypanosoma brucei.Nature reviews. Microbiology · 2014Review
- New insights into the molecular mechanisms of mitosis and cytokinesis in trypanosomes.International review of cell and molecular biology · 2014Review
- Identification of paralogous life-cycle stage specific cytoskeletal proteins in the parasite Trypanosoma brucei.PloS one · 2014Article
- The limits on trypanosomatid morphological diversity.PloS one · 2013Article
- Determinants of GPI-PLC localisation to the flagellum and access to GPI-anchored substrates in trypanosomes.PLoS pathogens · 2013Article
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5 authors.
Funding
Abstract
Flagellar attachment is a visibly striking morphological feature of African trypanosomes but little is known about the requirements for attachment at a molecular level. This study characterizes a previously undescribed membrane protein, FLA3, which plays an essential role in flagellar attachment in Trypanosoma brucei. FLA3 is heavily N-glycosylated, locates to the flagellar attachment zone and appears to be a bloodstream stage specific protein. Ablation of the FLA3 mRNA rapidly led to flagellar detachment and a concomitant failure of cytokinesis in the long slender bloodstream form but had no effect on the procyclic form. Flagellar detachment was obvious shortly after induction of the dsRNA and the newly synthesized flagellum was often completely detached after it emerged from the flagellar pocket. Within 12 h most cells possessed detached flagella alongside the existing attached flagellum. These results suggest that proteins involved in attachment are not shared between the new and old attachment zones. In other respects the detached flagella appear normal, they beat rapidly although directional motion was lost, and they possess an apparently normal axoneme and paraflagellar rod structure. The flagellar attachment zone appeared to be disrupted when FLA3 was depleted. Thus, while flagellar attachment is a constitutive feature of the life cycle of trypanosomes, attachment requires stage specific elements at the protein level.
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