ArticleApplied and environmental microbiology2013
wksl3, a New biocontrol agent for Salmonella enterica serovars enteritidis and typhimurium in foods: characterization, application, sequence analysis, and oral acute toxicity study.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Genotoxicity and sub-chronic oral toxicity of a blend of bacteriophage for animal feeds.Toxicology reports · 2026Article
- Formulation-Level Tolerability of Repeated Drinking-Water Administration of a Commercial Multi-Component Bacteriophage Product in Ross 308 Broiler Chickens: A Pilot 27-Day Dose-Escalation Study.Veterinary sciences · 2026Article
- A Comprehensive Review of the Application of Bacteriophages Against Enteric Bacterial Infection in Poultry: Current Status, Challenges, and Future Prospects.Antibiotics (Basel, Switzerland) · 2025Review
- Isolation, whole genome sequencing and application of a broad-spectrum Salmonella phage.Archives of microbiology · 2024Article
- Multireceptor phage cocktail againstmicroLife · 2024Article
- An Overview of the Public Health Challenges in Diagnosing and Controlling Human Foodborne Pathogens.Vaccines · 2023Review
- Comparative Genomics of a PolyvalentViruses · 2023Article
- Application of a novel lyticFrontiers in microbiology · 2023Article
- Prevention and Control of HumanInternational journal of food science · 2023Review
- The Use of Natural Methods to Control Foodborne Biofilms.Pathogens (Basel, Switzerland) · 2022Review
- Recent Advances in the Application of Bacteriophages against Common Foodborne Pathogens.Antibiotics (Basel, Switzerland) · 2022Review
- Bacteriophages as Biocontrol Agents in Livestock Food Production.Microorganisms · 2022Review
- The Combination of Salmonella Phage ST-3 and Antibiotics to Prevent Salmonella Typhimurium In Vitro.Current microbiology · 2022Article
- NovelMicroorganisms · 2022Article
- Oral Toxicity Study for Salmonella Killing Lytic Bacteriophage NINP13076 in BALB/c Mice and Its Effect on Probiotic Microbiota.Current microbiology · 2022Article
- Therapeutic Bacteriophages for Gram-Negative Bacterial Infections in Animals and Humans.Pathogens & immunity · 2022Review
- The Lytic Siphophage vB_StyS-LmqsSP1 Reduces the Number of Salmonella enterica Serovar Typhimurium Isolates on Chicken Skin.Applied and environmental microbiology · 2021Article
- Genomic and functional characterization of five novel Salmonella-targeting bacteriophages.Virology journal · 2021Article
- Phages and Enzybiotics in Food Biopreservation.Molecules (Basel, Switzerland) · 2021Review
- Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug ResistantMicroorganisms · 2021Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Of the Salmonella enterica serovars, S. Enteritidis and S. Typhimurium are responsible for most of the Salmonella outbreaks implicated in the consumption of contaminated foods in the Republic of Korea. Because of the widespread occurrence of antimicrobial-resistant Salmonella in foods and food processing environments, bacteriophages have recently surfaced as an alternative biocontrol tool. In this study, we isolated a virulent bacteriophage (wksl3) that could specifically infect S. Enteritidis, S. Typhimurium, and several additional serovars. Transmission electron microscopy revealed that phage wksl3 belongs to the family Siphoviridae. Complete genome sequence analysis and bioinformatic analysis revealed that the DNA of phage wksl3 is composed of 42,766 bp with 64 open reading frames. Since it does not encode any phage lysogeny factors, toxins, pathogen-related genes, or food-borne allergens, phage wksl3 may be considered a virulent phage with no side effects. Analysis of genetic similarities between phage wksl3 and four of its relatives (SS3e, vB_SenS-Ent1, SE2, and SETP3) allowed wksl3 to be categorized as a SETP3-like phage. A single-dose test of oral toxicity with BALB/c mice resulted in no abnormal clinical observations. Moreover, phage application to chicken skin at 8°C resulted in an about 2.5-log reduction in the number of Salmonella bacteria during the test period. The strong, stable lytic activity, the significant reduction of the number of S. Enteritidis bacteria after application to food, and the lack of clinical symptoms of this phage suggest that wksl3 may be a useful agent for the protection of foods against S. Enteritidis and S. Typhimurium contamination.
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