ArticleApplied and environmental microbiology2013
Cloning of a novel nicotine oxidase gene from Pseudomonas sp. strain HZN6 whose product nonenantioselectively degrades nicotine to pseudooxynicotine.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Insights into Pharmacological Activities of Nicotine and 6-Hydroxy-L-nicotine, a Bacterial Nicotine Derivative: A Systematic Review.Biomolecules · 2023Pooled it
- Additional Role of Nicotinic Acid Hydroxylase for the Transformation of 3-Succinoyl-Pyridine byApplied and environmental microbiology · 2021Article
- Article
- Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822.BMC genomics · 2019Article
- The enzymes of microbial nicotine metabolism.Beilstein journal of organic chemistry · 2018Review
- Characterization of the ModABC Molybdate Transport System ofFrontiers in microbiology · 2018Article
- Characterization and Genome Analysis of a Nicotine and Nicotinic Acid-Degrading Strain Pseudomonas putida JQ581 Isolated from Marine.Marine drugs · 2017Article
- Comparative Genomics Reveals Specific Genetic Architectures in Nicotine Metabolism ofFrontiers in microbiology · 2017Article
- Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineeredBiotechnology for biofuels · 2017Article
- Mechanism of the Flavoprotein L-Hydroxynicotine Oxidase: Kinetic Mechanism, Substrate Specificity, Reaction Product, and Roles of Active-Site Residues.Biochemistry · 2016Article
- Nicotine Dehydrogenase Complexed with 6-Hydroxypseudooxynicotine Oxidase Involved in the Hybrid Nicotine-Degrading Pathway in Agrobacterium tumefaciens S33.Applied and environmental microbiology · 2016Article
- Comparative genome analysis reveals the molecular basis of nicotine degradation and survival capacities of Arthrobacter.Scientific reports · 2015Article
- Molecular mechanism of nicotine degradation by a newly isolated strain, Ochrobactrum sp. strain SJY1.Applied and environmental microbiology · 2015Article
- Microbial community degradation of widely used quaternary ammonium disinfectants.Applied and environmental microbiology · 2014Article
- A novel (S)-6-hydroxynicotine oxidase gene from Shinella sp. strain HZN7.Applied and environmental microbiology · 2014Article
- Genome Sequence of a Newly Isolated Nicotine-Degrading Bacterium, Ochrobactrum sp. SJY1.Genome announcements · 2014Article
- Systematic unraveling of the unsolved pathway of nicotine degradation in Pseudomonas.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas sp. strain HZN6 utilizes nicotine as its sole source of carbon, nitrogen, and energy. However, its catabolic mechanism has not been elucidated. In this study, self-formed adaptor PCR was performed to amplify the upstream sequence of the pseudooxynicotine amine oxidase gene. A 1,437-bp open reading frame (designated nox) was found to encode a nicotine oxidase (NOX) that shows 30% amino acid sequence identity with 6-hydroxy-l-nicotine oxidase from Arthrobacter nicotinovorans. The nox gene was cloned into a broad-host-range cloning vector and transferred into the non-nicotine-degrading bacteria Escherichia coli DH5α (DH-nox) and Pseudomonas putida KT2440 (KT-nox). The transconjugant KT-nox obtained nicotine degradation ability and yielded an equimolar amount of pseudooxynicotine, while DH-nox did not. Reverse transcription-PCR showed that the nox gene is expressed in both DH5α and KT2440, suggesting that additional factors required for nicotine degradation are present in a Pseudomonas strain(s), but not in E. coli. The mutant of strain HZN6 with nox disrupted lost the ability to degrade nicotine, but not pseudooxynicotine. These results suggested that the nox gene is responsible for the first step of nicotine degradation. The (RS)-nicotine degradation results showed that the two enantiomers were degraded at approximately the same rate, indicating that NOX does not show chiral selectivity. Site-directed mutagenesis revealed that both the conserved flavin adenine dinucleotide (FAD)-binding GXGXXG motif and His456 are essential for nicotine degradation activity.
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