ArticleJournal of bacteriology2013
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Structural and functional analysis of themBio · 2026Article
- Rapid identification platform of spores based on Raman spectroscopy.Food chemistry: X · 2025Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.Genes & development · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Dormant spores sense amino acids through the B subunits of their germination receptors.Nature communications · 2021Article
- Dynamics of Germinosome Formation and FRET-Based Analysis of Interactions between GerD and Germinant Receptor Subunits inInternational journal of molecular sciences · 2021Article
- Clostridioides difficile SpoVAD and SpoVAE Interact and Are Required for Dipicolinic Acid Uptake into Spores.Journal of bacteriology · 2021Article
- Predicting the Structure and Dynamics of Membrane Protein GerAB fromInternational journal of molecular sciences · 2021Article
- Structural and functional analyses of the N-terminal domain of the A subunit of aProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Membrane Proteomes and Ion Transporters inJournal of bacteriology · 2019Article
- Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis.PloS one · 2018Article
- Diversity of the Germination Apparatus inFrontiers in microbiology · 2016Article
- Identification of a Novel Lipoprotein Regulator of Clostridium difficile Spore Germination.PLoS pathogens · 2015Article
- The effects of heat activation on Bacillus spore germination, with nutrients or under high pressure, with or without various germination proteins.Applied and environmental microbiology · 2015Article
- Article
- HtrC is involved in proteolysis of YpeB during germination of Bacillus anthracis and Bacillus subtilis spores.Journal of bacteriology · 2015Article
- Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.Journal of bacteriology · 2015Article
- Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores.Journal of bacteriology · 2014Article
- Structural and functional analysis of the GerD spore germination protein of Bacillus species.Journal of molecular biology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Access to a membrane-impermeant biotinylation reagent as well as protease sensitivity was used to determine germination proteins' topology in the inner membrane (IM) of decoated dormant spores and intact germinated Bacillus subtilis spores. The proteins examined were four nutrient germinant receptor (GR) subunits, the GerD protein, essential for normal GR-dependent spore germination, the SpoVAD protein, essential for dipicolinic acid movement across the IM, the SleB cortex-lytic enzyme, and the YpeB protein, essential for SleB assembly in spores, as well as green fluorescent protein (GFP) in the spore core. GerD and SpoVAD as well as GFP in the spore were not biotinylated in decoated dormant spores. However, GR subunits, SleB, and YpeB were biotinylated 4 to 36% in decoated dormant spores, although these levels were not increased by higher biotinylation reagent concentrations or longer reaction times. In contrast, the germination proteins were largely biotinylated in germinated spores, although GFP was not. All of the germination proteins in the germinated spore's IM, but not spore core GFP, were largely sensitive to an exogenous protease. These results, coupled with predicted or experimentally determined structural data, indicate that (i) these germination proteins are at least partially and in some cases completely on the outer surface of the spore's IM and (ii) there is significant reorganization of these germination proteins' structure or environment in the IM during spore germination.
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