ArticleJournal of bacteriology2013
Functional analysis of a class I holin, P2 Y.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- The Lysis cassette of jumbophage PhiKZ.Scientific reports · 2026Article
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- The Lysis Cassette of Jumbophage phiKZ.bioRxiv : the preprint server for biology · 2025Article
- Genomic analysis of Salmonella bacteriophages revealed multiple endolysin ORFs and importance of ligand-binding site of receptor-binding protein.FEMS microbiology ecology · 2024Article
- Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications.Protein and peptide letters · 2024Review
- A dimeric holin/antiholin complex controls lysis by phage T4.Frontiers in microbiology · 2024Article
- Endolysin Regulation in Phage Mu Lysis.mBio · 2022Article
- Article
- Localization and Regulation of the T1 Unimolecular Spanin.Journal of virology · 2018Article
- Enzymes and Mechanisms Employed by Tailed Bacteriophages to Breach the Bacterial Cell Barriers.Viruses · 2018Review
- The temperate Burkholderia phage AP3 of the Peduovirinae shows efficient antimicrobial activity against B. cenocepacia of the IIIA lineage.Applied microbiology and biotechnology · 2017Article
- The Last r Locus Unveiled: T4 RIII Is a Cytoplasmic Antiholin.Journal of bacteriology · 2016Article
- Complete Genome Sequence of Citrobacter freundii Myophage Michonne.Genome announcements · 2015Article
- Probing the structure of the S105 hole.Journal of bacteriology · 2014Article
- Genetic dissection of T4 lysis.Journal of bacteriology · 2014Article
- Stable micron-scale holes are a general feature of canonical holins.Molecular microbiology · 2014Article
- Bacteriophage P2.BacteriophageReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Y is the putative holin gene of the paradigm coliphage P2 and encodes a 93-amino-acid protein. Y is predicted to be an integral membrane protein that adopts an N-out C-in membrane topology with 3 transmembrane domains (TMDs) and a highly charged C-terminal cytoplasmic tail. The same features are observed in the canonical class I lambda holin, the S105 protein of phage lambda, which controls lysis by forming holes in the plasma membrane at a programmed time. S105 has been the subject of intensive genetic, cellular, and biochemical analyses. Although Y is not related to S105 in its primary structure, its characterization might prove useful in discerning the essential traits for holin function. Here, we used physiological and genetic approaches to show that Y exhibits the essential holin functional criteria, namely, allele-specific delayed-onset lethality and sensitivity to the energization of the membrane. Taken together, these results suggest that class I holins share a set of unusual features that are needed for their remarkable ability to program the end of the phage infection cycle with precise timing. However, Y holin function requires the integrity of its short cytoplasmic C-terminal domain, unlike for S105. Finally, instead of encoding a second translational product of Y as an antiholin, as shown for lambda S107, the P2 lysis cassette encodes another predicted membrane protein, LysA, which is shown here to have a Y-specific antiholin character.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.