ArticleAdvanced healthcare materials2013
Surface-micromachined microfiltration membranes for efficient isolation and functional immunophenotyping of subpopulations of immune cells.
Article in Advanced healthcare materials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
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- A Microfluidic Platform Revealing Interactions between Leukocytes and Cancer Cells on Topographic Micropatterns.Biosensors · 2022Article
- Ultrasensitive Multiparameter Phenotyping of Rare Cells Using an Integrated Digital-Molecular-Counting Microfluidic Well Plate.Small (Weinheim an der Bergstrasse, Germany) · 2021Article
- Pancreas-on-a-Chip Technology for Transplantation Applications.Current diabetes reports · 2020Review
- Mass-producible microporous silicon membranes for specific leukocyte subset isolation, immunophenotyping, and personalized immunomodulatory drug screening in vitro.Lab on a chip · 2019Article
- An integrated adipose-tissue-on-chip nanoplasmonic biosensing platform for investigating obesity-associated inflammation.Lab on a chip · 2018Article
- A fluorescent microbead-based microfluidic immunoassay chip for immune cell cytokine secretion quantification.Lab on a chip · 2018Article
- Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells.Bioengineering (Basel, Switzerland) · 2017Article
- Surface micromachining of polydimethylsiloxane for microfluidics applications.Biomicrofluidics · 2016Article
- Microfluidics: A new tool for modeling cancer-immune interactions.Trends in cancer · 2016Article
- Micro- and nanodevices integrated with biomolecular probes.Biotechnology advances · 2015Review
- Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA.Scientific reports · 2015Article
- Benchtop technologies for circulating tumor cells separation based on biophysical properties.BioMed research international · 2015Review
- Emerging microengineered tools for functional analysis and phenotyping of blood cells.Trends in biotechnology · 2014Review
- Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes.Lab on a chip · 2014Article
- Microfluidic blood cell sorting: now and beyond.Small (Weinheim an der Bergstrasse, Germany) · 2014Review
- Integrated nanoplasmonic sensing for cellular functional immunoanalysis using human blood.ACS nano · 2014Article
- Micropatterned photodegradable hydrogels for the sorting of microbeads and cells.Angewandte Chemie (International ed. in English) · 2013Article
- Emerging microfluidic tools for functional cellular immunophenotyping: a new potential paradigm for immune status characterization.Frontiers in oncology · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
An accurate measurement of the immune status in patients with immune system disorders is critical in evaluating the stage of diseases and tailoring drug treatments. The functional cellular immunity test is a promising method to establish the diagnosis of immune dysfunctions. The conventional functional cellular immunity test involves measurements of the capacity of peripheral blood mononuclear cells to produce pro-inflammatory cytokines when stimulated ex vivo. However, this "bulk" assay measures the overall reactivity of a population of lymphocytes and monocytes, making it difficult to pinpoint the phenotype or real identity of the reactive immune cells involved. In this research, we develop a large surface micromachined poly-dimethylsiloxane (PDMS) microfiltration membrane (PMM) with high porosity, which is integrated in a microfluidic microfiltration platform. Using the PMM with functionalized microbeads conjugated with antibodies against specific cell surface proteins, we demonstrated rapid, efficient and high-throughput on-chip isolation, enrichment, and stimulation of subpopulations of immune cells from blood specimens. Furthermore, the PMM-integrated microfiltration platform, coupled with a no-wash homogeneous chemiluminescence assay ("AlphaLISA"), enables us to demonstrate rapid and sensitive on-chip immunophenotyping assays for subpopulations of immune cells isolated directly from minute quantities of blood samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.