ArticleBlood2013
The Rac GTPase effector p21-activated kinase is essential for hematopoietic stem/progenitor cell migration and engraftment.
Article in Blood, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Gadd45g insufficiency drives the pathogenesis of myeloproliferative neoplasms.Nature communications · 2024Article
- Adverse clinical outcomes and immunosuppressive microenvironment of RHO-GTPase activation pattern in hepatocellular carcinoma.Journal of translational medicine · 2024Article
- Molecular mechanisms of microRNA-216a during tumor progression.Cancer cell international · 2023Review
- p21-Activated kinases as promising therapeutic targets in hematological malignancies.Leukemia · 2022Review
- Group I p21-activated kinases in leukemia cell adhesion to fibronectin.Cell adhesion & migration · 2021Article
- The tetraspanin CD82 regulates bone marrow homing and engraftment of hematopoietic stem and progenitor cells.Molecular biology of the cell · 2018Article
- Combined inhibition of receptor tyrosine and p21-activated kinases as a therapeutic strategy in childhood ALL.Blood advances · 2018Article
- A key role for Rac and Pak signaling in neutrophil extracellular traps (NETs) formation defines a new potential therapeutic target.American journal of hematology · 2018Article
- Pak2 regulates myeloid-derived suppressor cell development in mice.Blood advances · 2017Article
- Prognostic and biological significance of the proangiogenic factor EGFL7 in acute myeloid leukemia.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Niche WNT5A regulates the actin cytoskeleton during regeneration of hematopoietic stem cells.The Journal of experimental medicine · 2017Article
- Article
- p21-Activated Kinase 2 Regulates Endothelial Development and Function through the Bmk1/Erk5 Pathway.Molecular and cellular biology · 2015Article
- Article
- Pak2 restrains endomitosis during megakaryopoiesis and alters cytoskeleton organization.Blood · 2015Article
- Pak2 regulates hematopoietic progenitor cell proliferation, survival, and differentiation.Stem cells (Dayton, Ohio) · 2015Article
- Beta-Chemokine CCL15 Affects the Adhesion and Migration of Hematopoietic Progenitor Cells.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2015Article
- Remarkable reductions of PAKs in the brain tissues of scrapie-infected rodent possibly linked closely with neuron loss.Medical microbiology and immunology · 2014Review
- SPARC promotes leukemic cell growth and predicts acute myeloid leukemia outcome.The Journal of clinical investigation · 2014Article
- PAK signalling during the development and progression of cancer.Nature reviews. Cancer · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
The p21-activated kinases (Paks) are serine/threonine kinases that are major effectors of the Rho guanosine 5'\x{2011}triphosphatase, Rac, and Cdc42. Rac and Cdc42 are known regulators of hematopoietic stem and progenitor cell (HSPC) function, however, a direct role for Paks in HSPCs has yet to be elucidated. Lin(-)Sca1(+)c-kit(+) (LSK) cells from wild-type mice were transduced with retrovirus expressing Pak inhibitory domain (PID), a well-characterized inhibitor of Pak activation. Defects in marrow homing and in vitro cell migration, assembly of the actin cytoskeleton, proliferation, and survival were associated with engraftment failure of PID-LSK. The PID-LSK demonstrated decreased phosphorylation of extracellular signal-regulated kinase (ERK), whereas constitutive activation of ERK in these cells led to rescue of hematopoietic progenitor cell proliferation in vitro and partial rescue of Pak-deficient HSPC homing and engraftment in vivo. Using conditional knock-out mice, we demonstrate that among group A Paks, Pak2(-/-) HSPC show reduced homing to the bone marrow and altered cell shape similar to PID-LSK cells in vitro and are completely defective in HSPC engraftment. These data demonstrate that Pak proteins are key components of multiple engraftment-associated HSPC functions and play a direct role in activation of ERK in HSPCs, and that Pak2 is specifically essential for HSPC engraftment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.